The particular interaction of each cement with dentin results in specific bond strength and failure patterns that varied among groups in both evaluation times. Even though the self-adhesive cement tested exhibited no authentic hybrid layer, it was able to promote reliable adhesion with the underlying dentin.

Despite studies concerning toxic reactions related to amalgam components in the literature, few studies have been devoted to evaluate local noxious effects of amalgam tattoos (AT) on biological tissues. In addition, little is known about activation of inflammatory cells by mucosa-implanted amalgam debris. Tissue reaction to AT depends on the particle size. Human leukocyte antigen DR (HLA-DR) is an activation marker of inflammatory cells associated with antigen presentation. Metallothioneins (MT) are proteins involved with metal detoxication, including mercury and silver. The purpose of the present study was to investigate the immunolocalization of HLA-DR and MT in AT with large or powdered particles. Paraffin-embedded AT tissue blocks were sectioned and subjected to immunohistochemistry for HLA-DR and MT localization. The results demonstrated a dense mononuclear inflammatory infiltrate associated with large and powdered debris and positivity for HLA-DR and MT in inflammatory cells. While blood vessel walls and connective fibers impregnated with powdered particles were negative for HLA-DR, they were positive for MT. In addition, wherever epithelial basement membrane impregnation by powdered amalgam particles was observed, a strong positivity for MT was detected. These findings demonstrate that residual elements of AT still have noxious local effects over tissues.

Within the limitations of this study, there was no significant difference in vertical misfit between the titanium and zirconia implant frameworks produced using the CAD/CAM system. Regardless of the material used, the CAD/CAM frameworks achieved an adequate vertical fit.

The present study shows that oral epithelial cells of BMT patients show increased immunolocalization of the DNA repair related proteins.

A DEUS, pela sua presença em minha vida e por sua infinita bondade, sem a qual nada seria possível.A minha esposa Rosane e aos meus filhos Victor e Laura pela compreensão, incentivo e amor. A participação e a solidariedade de vocês foram fundamentais para conclusão deste ideal.A memória dos meus pais Renato e Maria Terezinha, exemplo de dedicação para a nossa família.