SUMMARYThe tissue changes that occur in Chagas disease are related to the degree of oxidative stress and antioxidant capacity of affected tissue. Studies with vitamin C supplementation did not develop oxidative damage caused by Chagas disease in the host, but other studies cite the use of peroxiredoxins ascorbate -dependent on T. cruzi to offer protection against immune reaction. Based on these propositions, thirty "Swiss" mice were infected with T. cruzi QM1 strain and treated with two different vitamin C doses in order to study the parasitemia evolution, histopathological changes and lipid peroxidation biomarkers during the acute phase of Chagas disease. The results showed that the parasite clearance was greater in animals fed with vitamin C overdose. There were no significant differences regarding the biomarkers of lipid peroxidation and inflammatory process or the increase of myocardium in animals treated with the recommended dosage. The largest amount of parasite growth towards the end of the acute phase suggests the benefit of high doses of vitamin C for trypomastigotes. The supplementation doesn't influence the production of free radicals or the number of amastigote nests in the acute phase of Chagas disease.
Introduction:In order to examine the effectiveness of vitamin C (ascorbic acid) in combating
the oxidative insult caused by Trypanosoma cruzi during the
development of the chronic phase of Chagas disease, Swiss mice were infected
intraperitoneally with 5.0 × 104 trypomastigotes of T.
cruzi QM1strain.Methods:Mice were given supplements of two different doses of vitamin C for 180 days.
Levels of lipid oxidation (as indicated by thiobarbituric acid reactive
substances-TBARS), total peroxide, vitamin C, and reduced glutathione were
measured in the plasma, TBARS, total peroxide and vitamin C were measured in the
myocardium and histopathologic analysis was undertaken in heart, colon and
skeletal muscle.Results:Animals that received a dose equivalent to 500 mg of vitamin C daily showed
increased production of ROS in plasma and myocardium and a greater degree of
inflammation and necrosis in skeletal muscles than those that received a lower
dose or no vitamin C whatsoever.Conclusion:Although some research has shown the antioxidant effect of vitamin C, the results
showed that animals subject to a 500 mg dose of vitamin C showed greater tissue
damage in the chronic phase of Chagas disease, probably due to the paradoxical
actions of the substance, which in this pathology, will have acted as a
pro-oxidant or pro-inflammatory.
The elaborated molds had identical size and shape of a human ear and could be positioned in the same shape of the patient ears. Thus, the synthetic molds were presented as promising simulation tools for the training and surgical enhancement of otoplasty, especially for doctors beginners.
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