Increasing the germination percentages from chemical and/or mechanical processes in native species is vital for the conservation of the species. The objective was to evaluate the effect of different treatments on the germination process of species belonging to the tropical deciduous forest (TDF) in a forest nursery. Eight species of trees and shrubs reported to be native to the TDF were selected; the seeds were collected from various sites of the state of Queretaro, Mexico. A randomized design of four replicates with 25 seeds was used, for which three pre-germinative scarification treatments were evaluated for each species: (1) thermal scarification (TS), (2) mechanical scarification (MS), and (3) chemical scarification (CS). Germination was considered as the protrusion of cotyledons onto the substrate and was recorded every 48 h for 60 days. A significant difference was observed between treatments (p ≤ 0.05). The mechanical treatment (MS) obtained the highest germination percentage and the least variation, in contrast to the thermal, chemical, and control treatments, which presented the lowest amount of germinated seeds. The MS treatment was the most effective for all the species studied because it allowed for 100% of the germination to be achieved.