<em>The objective of research was to evaluate the effect of different level of decomposers from bioethanol waste as a bioactivator to compost nutrient content. The materials used in this study were feces collected from six 3 to 5 years old of male Fresian Holstein (PFH), and decomposer agents from processed-bioethanol waste. The experimental design used was completely randomized design with four treatments and six replications, i.e. P1: 0.4% of decomposer, P2: 0.5% of decomposer, P3: 0.6% of decomposer, P4: 0.7 of decomposer %. Analysis of nutrient contents of compost was conducted at the the Laboratory of Chemistry and Soil Fertility, Department of Soil Science, Faculty of Agriculture, Gajah Mada University, Yogyakarta. Data analysis was performed using Minitab 17 software. The results of the analysis of variance showed that different levels of decomposer affected organic C, phosphor (P), Kalium (K), and Magnesium (Mg). </em><em>Organic C, P, K, and Mg contents were found highest in P2, on the other hand N and Ca contents, C/N ratio, pH, and temperature of compost were not different among treatments. It could be concluded that compost made with 0.5% decomposer agents from processed-bioethanol waste was the best.</em>
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