Skim is a major by-product of the virgin coconut oil (VCO) industry consisting of nutrients and is currently discharged to the environment as waste. Hence, this research aimed to utilize skim as a medium for Botryococcus braunii and Chlorella vulgaris growth. The effect of organic carbon resourced from glucose and glycerol on biomass was also investigated at certain concentrations with intervals 100:0 to 0:100 (%). Results indicated that Botryococcus braunii and Chlorella vulgaris cultivated under 20% VCO mill effluent gave the highest biomass production, which accounts for 1.69 g/L and 5.34 g/L; 89.40% and 86.70% COD reduction efficiency. Whereas, mixotrophic condition showed a positive trend for Botryococcus braunii at 80:20 (glucose:glycerol) with 5.60 g/L biomass production and 97.64% COD removal efficiency. Vice versa, the addition of glucose:glycerol gave an inhibitory impact on Chlorella vulgaris as the biomass production was merely 1.66 g/L with COD efficiency 94.03%.
Salah satu komoditas perikanan andalan Indonesia adalah ikan tuna (Thunnus sp.) yang diekspor dalam berbagai bentuk olahan ke pasar ekspor internasional. Namun, kasus penolakan produk ikan tuna dari pasar ekspor saat ini cukup besar. Permasalahan utama kasus penolakan tersebut adalah kandungan bakteri atau kadar histamin yang tinggi pada produk. Oleh sebab itu, penelitian ini dilakukan untuk mengkaji pengaruh suhu penerimaan sampel dan bentuk olahan ikan tuna terhadap kandungan histamin. Sampel ikan tuna telah diuji menggunakan metode Enzyme Linked Immunosorbent Assay (ELISA) terhadap perubahan suhu penerimaan sampel ikan dengan rentang suhu -0,1 sampai -0,5°C dan olahan tuna bentuk steak dan loin. Dari hasil penelitian, keseluruhan sampel ikan tuna mengandung kadar histamin <1 ppm. Perubahan suhu penerimaan sampel tidak memberikan perbedaan hasil yang besar terhadap kandungan histamin dengan konsentrasi akhir 0,4 ppm. Bentuk loin dan steak pada sampel juga memiliki rata-rata kadar histamin yang tidak jauh berbeda, yaitu 0,6 dan 0,3 ppm.
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