Aim and objectives: To compare and evaluate the effect of sample type on vitamin D concentration in order to avoid multiple sample collection. Materials and methods: The study was conducted in a tertiary care hospital on 40 adult subjects whose samples were for both serum vitamin D and plasma parathyroid hormone (PTH). Leftover plasma sample was utilized for vitamin D analysis. Samples were analyzed on Advia Centaur XP from Siemens Healthineers. Bland-Altman analysis and regression equation were derived to evaluate the extent of agreement and conversion between sample types. Results and conclusion: Serum vitamin D is higher than plasma vitamin D (by 6.5 ng/mL), and it is more pronounced in samples with vitamin D greater than 20 ng/mL. Clinical significance: It is important that lab and clinicians should be aware of the comparably large preanalytical bias introduced by changing between serum and EDTA plasma sample for vitamin D analysis. This would impact patients who are serially monitored for vitamin D supplementation.
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