Background: STMS markers and morphological traits were used to investigate the genetic relationship and allelic diversity in chickpea. In this study, we focused on the selection and more efficient utilization of core germplasm in breeding programs for chickpea crop improvement using STMS and quantitative / morphological traits.
Methods: Seeds of elite accessions of chickpea were obtained from ICRISAT, Patancheru, Andhra Pradesh, India. 50 STMS markers and 11 quantitative traits were used for exploring the genetic variability and relationship in 35 chickpea accessions.
Result: A total of 97 alleles were produced out of the 32 polymorphic STMS loci with an average of 3.03 alleles per locus ranging between 2-6 alleles per primer. The PIC value ranged from 0.029 to 0.768 with an average of 0.502. PIC value showed a highly positive correlation (r = 0.718) with number of alleles at the STMS loci. In both molecular and morphological markers / traits-based clustering, out of 35 chickpea accessions only one accession ICC-13892 was isolated at the end of clustering. The results indicated that highly polymorphic microsatellite markers NCPGR 68, NCPGR 50, NCPGR 81, NCPGR 48 and NCPGR 77 along with the accessions ICC-13892 having distant associations with ICC-13816, ICC-15697, ICC-15610, ICC-15868, ICC-15888, ICC-15996 with novel findings should be useful resources for strategies of allele mining, association genetics, mapping and cloning of gene(s) and in applied breeding to broaden the genetic base of chickpea.
Background: United Nations SDG-2 and SDG-3 pledges to end hunger, achieve food security, improve nutrition and reduce premature deaths of children persisting amongst 821 million people globally. Chickpeas, the second largest food legume in the world, are important due to dietary, therapeutic and health values since Roman times and are ideal source of protein, carbohydrate, minerals and β-carotene. Therefore, it is central to alleviate malnutrition and ensuring good health.Methods: We conducted experiments during Rabi crop season 2018-19 and 2019-20 and studied proximate nutritional compositions such as protein, its bioavailability enhancement procedures, optimum sprouting stage and potential genotypes. The present series experiment as 6th one was undertaken to find out the impacts of varying seed seedlings germination stages at 0, 3 and 6 days on observed estimates of protein contents in 12 potential genotypes of chickpea including Pusa-256 as standard (control) check.Result: We noticed erosion of reserved seed nutrients activating seedling growth and enhancing nutritional values, observed protein in the range of 18.96 to 28.69%, discovered 6 days sprouts giving highest digestible protein and identified genotypes BG-1092, ICC-11378, JG-74 as potential resources to be utilized in breeding programmes for harnessing their genetic potentials for enriching protein and other nutritional components.
INTRODUCTIONHeterocycles predominantly containing nitrogen have been renowned as a class of auspicious and handy structures for the novel tetrazole chromophore design (e.g., pyridine tetrazole, bis-tetrazole pyridine, bipyridine tetrazole and terpyridine tetrazole ligands) [1][2][3][4][5][6][7] and synthesis of the elevated energy materials. Due to their higher thermal stability, higher heat of formation of these pyridine based derivatives found much imperative applications embracing the improvement of photoluminescent materials [8], protein crystallography [9] and immense variety of many other applications ranging from material science to medicinal field [10][11][12]. One of the most imperative and sensational applications of tetrazole based derivative as the pyridine tetrazole, which has been set up to display extensive appliances particularly in luminescent applications such as indicators, imaging and signal sensors [13,14].These tetrazole based derivatives can be used as a ligand in the configuration of eclectic range of complexes liable upon the nature of the central metal. Recently, lanthanide complexes equipped, which has publicized much more boosted photo luminescent property [15]. Correspondingly, the complexes
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