Sesame (Sesamum indicum) is one of the oldest and most traditional oilseed crops, valued for its highquality seed oil. This culture was introduced in Burkina Faso at the beginning of the 20th century where it occupies a place of cash crop. The majority of sesame produced in Burkina Faso is export oriented. However, the contamination by Salmonella remains an unsolved problem. This retrospective study was carried out in order to assess the microbiological safety of sesame samples received at the Laboratoire National de Santé Publique between 2007 and 2017. Out of 359 samples unevenly distributed according to months, 26.46% showed the presence of Salmonella species. The persistence of this food borne pathogen in sesame is a strong signal that new strategies of growing, harvesting and postharvest and special attention and emphasis on control measures must be given to the chain of production of this commodity in a view of its economic and medical impact.
With high annual mortality rates among young children, antimicrobial resistant salmonellosis is considered a major public health concern worldwide. Antimicrobial resistant salmonellosis is a worldwide health issue, particularly in low income countries with high microbially-derived food contaminations. As a result, it is important to better understand the biological factors that may control these bacteria's dissemination low immunity individuals such as children. Thus, a sound epidemiological surveillance and control of salmonellosis (that is, tet and catA1) requires a better understanding of the role that class 1, 2 and 3 integrons play in the spread of these antimicrobial resistant genes. A total of 275 stool samples of children suffering of diarrhea in rural Burkina Faso were collected and their Salmonella species were screened. The antimicrobial resistance determinants were investigated by Polymerase Chain Reaction, checking the presence of class 1, 2, 3 integrons, tet and catA1 resistance genes. Seven of the nine confirmed Salmonella strains (78%) were multidrug resistant while 100% were resistant to amoxicillin. Antibiotic resistance genes catA and tet were present in 11.1 and 22.2%, respectively. Integrons were detected as follows: Int1 (44.4%) and Int2 (22.2%). No class 3 integron was detected. A surveillance and control programme of antimicrobial drug resistant Salmonella species is of paramount importance for limiting spread of these pathogens among children.
Campylobacter is a major agent of gastroenteritis worldwide. The incidence and prevalence of campylobacteriosis have been increasing in both developed and developing countries over the last decade. In this study, 197 strains of successive Campylobacter-like were identified in French laboratories in September 2018. Bacterial isolates from clinical samples were identified with a mass spectrometer (Ultraflex III TOF/TOF and the BIOTYPER database from Bruker Daltonics). Of the 197 isolates tested, 143 were identified as Campylobacter jejuni (72.59%), 28 as Campylobacter coli (14.21%), 2 as Arcobacter butzleri (1.02%), 1 as Campylobacter fetus and 1 as Campylobacter lari with (0.51% each) by the MALDI-TOF mass spectrometry. Isolation rate of Campylobacter was highest in the 0 -9 age group (22%). The proportion of male and female patients was 59.4% (CI 95% = 52.2-66.3) and 40.6% (CI 95% = 33.7-42.8) respectively. Sixty strains (30.5%) were resistant to tetracycline and 52 (26.4%) resistant to ampicillin. This study showed that the MALDI-TOF mass spectrometry is a rapid and accurate identification method of Campylobater spp in patients treated in private French laboratories.
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