Phosphatidylinositol 4-kinase␤ (PI4K␤) plays an essential role in maintaining the structural integrity of the Golgi complex. In a search for PI4K␤-interacting proteins, we found that PI4K␤ specifically interacts with the GTP-bound form of the small GTPase rab11. The PI4K␤-rab11 interaction is of functional significance because inhibition of rab11 binding to PI4K␤ abolished the localization of rab11 to the Golgi complex and significantly inhibited transport of vesicular stomatitis virus G protein from the Golgi complex to the plasma membrane. We propose that a novel function of PI4K␤ is to act as a docking protein for rab11 in the Golgi complex, which is important for biosynthetic membrane transport from the Golgi complex to the plasma membrane.