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Root P. speciosa † Instability index < 40 and > 40 is predicted the protein as stable and unstable, respectively. ‡ Aliphatic index (alanine, valine, isoleucine, and leucine) is regarded as a positive factor for the increase of thermostability of globular proteins.$ GRAVY is calculated as the sum of hydropathy values of all the amino acids, divided by the number of residues in the sequence. Negative and positive GRAVY values indicate that the protein is non-polar and polar, respectively.