Experimental methods are indispensable for the study of the function of biological macromolecules, not just as static structures, but as dynamic systems that change conformation, bind partners, perform reactions, and respond to different stimulus. However, providing a detailed structural interpretation of the results is often a very challenging task. While experimental and computational methods are often considered as two different and separate approaches, the power and utility of combining both is undeniable. The integration of the experimental data with computational techniques can assist and enrich the interpretation, providing new detailed molecular understanding of the systems. Here, we briefly describe the basic principles of how experimental data can be combined with computational methods to obtain insights into the molecular mechanism and expand the interpretation through the generation of detailed models.

The E1B 55kDa produced by human adenovirus type 5 is a multifunctional protein that participates in the regulation of several steps during the viral replication cycle. Previous studies suggest this protein plays an important role in postranscriptional regulation of viral and cellular gene expression, as it is required for the selective accumulation of maximal levels of viral late mRNA in the cytoplasm of the infected cell; however the molecular mechanisms that are altered or regulated by this protein have not been elucidated. A ribonucleoprotein motif that could implicate the direct interaction of the protein with RNA was initially predicted and tested in vitro , but the interaction with RNA could not be detected in infected cells, suggesting the interaction may be weak or transient. Here it was determined that the E1B 55kDa interacts with RNA in the context of the viral infection in non-transformed human cells, and its contribution to the adenovirus replication cycle was evaluated. Using recombinant adenoviruses with amino acid substitutions or a deletion in the ribonucleoprotein motif the interaction of E1B 55kDa with RNA was found to correlate with timely and efficient viral DNA replication and viral late mRNA accumulation and splicing.

Microsatellite variability of the wood stork Mycteria americana (Aves, Ciconiidae) in Cuba: implications for its conservation. Mycteria americana (Aves, Ciconiidae) is the only species of stork found in the Caribbean. It is a permanent yet rare resident in Cuba, with only two reproductively active colonies. In this work, we used five microsatellite loci to characterize 37 individuals from these colonies, located in two of the most important wetlands of Cuba, the Zapata Swamp and the Sabana-Camagüey Archipelago. We found low genetic variability, with similar values to those reported for North and South American populations of the species, and little but significant genetic differentiation between colonies. Our results highlight the need to improve the management and conservation planning of the species in Cuba because the combination of low genetic variation, small colonies, anthropogenic influence and climatic factors could threaten its persistence.

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