Myxosporeans are well-known parasites infecting food fishes in fresh and marine water around the globe. Grass carp (Ctenopharyngodon idella), a freshwater food fish commonly cultured in India with has significant economic importance. Herein, the study focuses on the description of a new myxosporean species, Myxobolus grassi sp. nov. from the gills as primary site and liver as secondary site of infection in grass carp. Both organs (gill and liver) were infected concurrently in the host and the prevalence of grass carp infection was 4.05% in gill filaments and liver, respectively. Identification of species was based on the morphological and morphometric features of the myxospore as well as 18S rDNA sequence data. A smear from gill and liver exhibited hundreds of morphologically similar myxospores. BLAST search revealed 98% sequence similarity and 0.03 genetic distance with M. catlae (KM029967) infecting gill lamellae of mrigal carp (Cirrhinus cirrhosus) from India and 98–84% sequence similarity with other myxobolids in India, China, Japan, Malaysia, Turkey and Hungary. Phylogenetically, it clustered with other myxobolids infecting gills and related organs (i.e., vital organ) of Indian cyprinid carp species. On the basis of myxospore morphology and 18S sequence, we propose M. grassi sp. nov.
The study presents the status of fish diversity, abundance and habitat structure of Surha Lake, which is a perennial and natural lake fed by the river Ganga. The study was conducted between 2011 to 2013 covering pre and post-monsoon seasons. In total, 4,852 individual fish specimens were collected representing 66 fish species belonging to 23 families. The species diversity comprised 65 species in pre-monsoon and 60 species in post-monsoon season (p<0.05). The highest species diversity was recorded for the family Cyprinidae (22), followed by Bagridae (7). An assessment of conservation status of 66 fish species as per IUCN Red List 2019 criteria listed 6 species under near threatened (NT), 54 under least concern (LC) and two species under vulnerable (VU) category. The study reports several commercially important species under near threatened (Chitala chitala, Labeo pangusia, Ompok bimaculatus, Ompok pabda, , Ailia coila and Bagarius bagarius), which makes Surha Taal an important natural lake from fishery management point of view. The study also prioritised different habitat types important for different species. The slow water in the mid-channel areas of the lake were categorised as important habitats for near threatened and vulnerable fish species like C. chitala, B. bagarius and Wallago attu, which can be prioritised for population level management.
A cell line was established from striped snakehead Channa striatus kidney. The cell line, named as SSK, has been passaged for over 62 times. Growth studies at different temperatures and foetal bovine serum (FBS) concentrations revealed that SSK cells show optimum growth at 28°C in L-15 medium containing 20% FBS. The chromosome number of SSK cells was 2n=40. Partial sequencing of mitochondrial cytochrome oxidase 1 gene of the cells confirmed that the cell line originated from C. striatus. The SSK cells were primarily epithelial, as determined using immunophenotyping. The cells from SSK cell line were transfected with phrGFP II-N mammalian expression vector. The SSK cell line was stored in liquid nitrogen (-196οC) at different passages and was successfully revived after four months of storage. The cell line could be successfullyemployed for cytotoxicity studies, as revealed by neutral red assay. The cell line can be a valuable surrogate to the whole fish studies.
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