Kombucha is a traditional beverage of sweetened black tea fermented with a symbiotic association of acetic acid bacteria and yeasts. In this study, kombucha fermented beverage (KFB) appeared to include nine chemical groups (alcohols, acids, lactones, condensed heterocyclic compounds, antibiotics, esters, aldehydes, fatty acids, and alkaloids) of many bioactive metabolites, as elucidated by gas chromatography–mass spectrometry (GC-MS) and IR spectra. The fermented metabolic components of KFB seem collectively to act in a synergistic action giving rise to the antimicrobial activity. Four types of kombucha preparations (fermented, neutralized, heat-treated and unfermented) were demonstrated with respect to their antimicrobial activity against some pathogenic bacterial and fungal strains using agar well diffusion assay. KFB exerted the strongest antimicrobial activities when compared with neutralized and heat-treated kombucha beverages (NKB and HKB). Staphylococcus aureus ATCC6538 (S. aureus) and Escherichia coli ATCC11229 (E. coli) were the organisms most susceptible to the antimicrobial activity of kombucha beverage preparations. Finally, the KFB preparation showed remarkable inhibitory activity against S. aureus and E. coli bacteria in a brain heart infusion broth and in some Egyptian fruit juices (apple, guava, strawberry, and tomato). These data reveal that kombucha is not only a prophylactic agent, but also appears to be promising as a safe alternative biopreservative, offering protection against pathogenic bacteria and fungi.
Kefir beverage (KB) is a fermented milk initiated by kefir grains rich with starter probiotics. The KB produced in this study seemed to contain many chemical compounds elucidated by gas chromatography–mass spectrometry (GC-MS) and IR spectra. These compounds could be classified into different chemical groups such as alcohols, phenols, esters, fatty esters, unsaturated fatty esters, steroids, polyalkenes, heterocyclic compounds and aromatic aldehydes. Both KB and neutralized kefir beverage (NKB) inhibited some pathogenic bacteria including Escherichia coli ATCC11229 (E. coli), Listeria monocytogenes ATCC 4957 (L. monocytogenes), Bacillus cereus ATCC 14579 (B. cereus), Salmonella typhimurium ATCC 14028 (Sal. typhimurium) as well as some tested fungal strains such as Aspergillus flavus ATCC 16872 (A. flavus) and Aspergillus niger ATCC 20611 (A. niger), but the inhibitory activity of KB was more powerful than that obtained by NKB. It also appeared to contain four lactic acid bacteria species, one acetic acid bacterium and two yeast species. Finally, the KB inhibited distinctively both S. aureus and Sal. typhimurium bacteria in a brain heart infusion broth and in some Egyptian fruit juices, including those made with apples, guava, strawberries and tomatoes.
The genus Aeromonas is widely distributed in aquatic environments and is recognized as a potential human pathogen. Some Aeromonas species are able to cause a wide spectrum of diseases, mainly gastroenteritis, skin and soft-tissue infections, bacteremia, and sepsis. The aim of the current study was to determine the prevalence of Aeromonas spp. in raw fish markets and humans in Zagazig, Egypt; identify the factors that contribute to virulence; determine the isolates’ profile of antibiotic resistance; and to elucidate the ability of Aeromonas spp. to form biofilms. The examined samples included fish tissues and organs from tilapia (Oreochromis niloticus, n = 160) and mugil (Mugil cephalus, n = 105), and human skin swabs (n = 51) and fecal samples (n = 27). Based on biochemical and PCR assays, 11 isolates (3.2%) were confirmed as Aeromonas spp. and four isolates (1.2%) were confirmed as A. hydrophila. The virulence genes including haemolysin (hyl A) and aerolysin (aer) were detected using PCR in A. hydrophila in percentages of 25% and 50%, respectively. The antimicrobial resistance of Aeromonas spp. was assessed against 14 antibiotics comprising six classes. The resistance to cefixime (81.8%) and tobramycin (45.4%) was observed. The multiple antibiotic resistance (MAR) index ranged between 0.142–0.642 with 64.2% of the isolates having MAR values equal to 0.642. Biofilm formation capacity was assessed using a microtiter plate assay, and two isolates (18.1%) were classified as biofilm producers. This study establishes a baseline for monitoring and controlling the multidrug-resistant Aeromonas spp. and especially A. hydrophila in marine foods consumed in our country to protect humans and animals.
A total of 185 food samples in Sharkia Governorate, Egypt were analyzed for the presence of Aeromonas spp. The recovered isolates were characterized by biochemical tests based on Bergey's Manual of Systematic Bacteriology and confirmed by API-20E kit. Of the samples analyzed, 56 (30.27 %) were positive for Aeromonas spp., with the highest incidence in raw milk (55 %) followed by Nile tilapia (48.6 %). A. hydrophila and A. salmonicida were the most predominantly isolated from raw milk and A. caviae was the most prevalent in raw lean beef. A variety of extracellular virulence factors were determined and 83.3 % exhibited β-hemolytic activity; 80 % exhibited lecithinase activity; 75 % exhibited protease activity and 90 % exhibited lipolytic activity. Furthermore, the cytotoxic (act) and the cytotonic (alt) enterotoxins were screened in three selected isolates using PCR assay and the data showed the detection of the two genes with predicted sizes of 232 bp and 442 bp fragments, respectively. These findings indicated a major health risk of infection with enterotoxigenic Aeromonas bacteria for people consuming raw and improper cooked food in Egypt.
ABSTRACT. The aim of present study was the uses of a series of α,β-unsaturated carbonyl compounds (chalcones), in the synthesis of pyridine, pyran, thiophene, thiazole, together with their uses in heterocyclic synthesis. The work has resulted in the synthesis of a variety of 2,5-dihydropyridine, hydrazide-hydrazone, thiophene derivatives, coumarin, pyran and thiazolo[4,5-d]thiazole derivatives. The antitumor activities of the newly synthesized products were carried out against three cancer cell lines namely MCF-7, NCI-H460 and SF-268 and normal human cell line WI38. In addition, the inhibitions of most of the synthesized compounds against c-Met kinase were studied and results showed that many compounds were of high inhibitions, and these are considered as promising anticancer agents. The results obtained encouraged further work in the future. KEY WORDS: Chalcones, Heterocyclic, Pyridine, Pyran, Thiophene, Thiazole, Antitumor Bull. Chem. Soc. Ethiop. 2022, 36(1), 149-172. DOI: https://dx.doi.org/10.4314/bcse.v36i1.13
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