The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. Yet there remain situations that argue for inclusion of more loci and increased discrimination. The PowerPlex(®) Fusion System allows simultaneous amplification of the following loci: Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, and D22S1045. The comprehensive list of loci amplified by the system generates a profile compatible with databases based on either the expanded CODIS or European Standard Set (ESS) requirements. Developmental validation testing followed SWGDAM guidelines and demonstrated the quality and robustness of the PowerPlex(®) Fusion System across a number of variables. Consistent and high-quality results were compiled using data from 12 separate forensic and research laboratories. The results verify that the PowerPlex(®) Fusion System is a robust and reliable STR-typing multiplex suitable for human identification.

The growing queue for DNA analysis in crime laboratories has prevented the analysis from providing investigative leads as turnaround time has grown, limiting the analytical results to a confirmatory role in the courtroom. Rapid DNA technology offers an opportunity to employ an automated system for the development of a DNA profile. The Rapid DNA technology permits a police booking station to take a buccal swab obtained from an arrestee, acquire a DNA profile, and test that profile against a DNA database, all while the arrestee remains in police custody during the booking process. Rapid DNA technologies are a capital-intensive system enabling sophisticated equipment designed for operation by individuals with limited technical training to provide investigative leads with immediate support. We present the testing of rapid DNA technology in a trial program conducted by the Kentucky State Police Forensic Laboratory. The Kentucky test confirms the efficacy of the rapid DNA testing as consistent with the findings from traditional laboratory testing. The economic analysis related to testing indicates that the time saving from the rapid DNA analysis yields benefits that far outweigh the costs from the change in technology.

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