The development of new biomaterials whose characteristics are as close as possible to the properties of living human tissues is one of the most promising areas of regenerative medicine. This work aimed at creating a bioplastic material based on collagen, elastin and hyaluronic acid and studying its structure and properties to assess the prospects for further use in clinical practice. Bioplastic material was obtained by mixing collagen, hyaluronic acid and elastin in predetermined proportions with distilled water. We treated the material with photochemical crosslinking to stabilize biofilm in a liquid medium and form a nanostructured scaffold. A commercial human skin fibroblast cell culture was used to assess the biomaterial cytotoxicity and biocompatibility. The visualization and studies of the biomaterial structure were performed using light and scanning electron microscopy. It has been shown that the obtained biomaterial is characterized by high resilience; it has also a high porosity. The co-culturing of the bioplastic material and human fibroblasts did not reveal any of its cytotoxic effects on cells in culture. It was shown that the biomaterial samples could maintain physical properties in the culture medium for more than 10 days, while the destruction of the matrix was observed 3–4 weeks after the beginning of incubation. Thus, the created biomaterial can be used on damaged skin areas due to its physical properties and structure. The use of the developed biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising wound cover for use in clinical practice.
The development of new biomaterials whose characteristics are as close as possible to the properties of living human tissues is one of the most promising areas of regenerative medicine. This work aimed at creating a bioplastic material based on collagen, elastin and hyaluronic acid and studying its structure and properties to assess the prospects for further use in clinical practice. Bioplastic material was obtained by mixing collagen, hyaluronic acid and elastin in predetermined proportions with distilled water. We treated the material with photochemical crosslinking to stabilize biofilm in a liquid medium and form a nanostructured scaffold. A commercial human skin fibroblast cell culture was used to assess the biomaterial cytotoxicity and biocompatibility. The visualization and studies of the biomaterial structure were performed using light and scanning electron microscopy. It has been shown that the obtained biomaterial is characterized by high resilience; it has also a high porosity. The co-culturing of the bioplastic material and human fibroblasts did not reveal any of its cytotoxic effects on cells in culture. It was shown that the biomaterial samples could maintain physical properties in the culture medium for more than 10 days, while the destruction of the matrix was observed 3–4 weeks after the beginning of incubation. Thus, the created biomaterial can be used on damaged skin areas due to its physical properties and structure. The use of the developed biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising wound cover for use in clinical practice.
The technique of obtaining a new bioplastic material based on collagen, elastin and hyaluronic acid is described. The results of a study of the biomaterial structure and properties in order to assess the prospects for its further use in clinical practice are presented. Co-culturing of the bioplastic material and human fibroblasts did not reveal any its cytotoxic effects on cells in culture. It was shown that the biomaterial samples were able to maintain physical properties in the culture medium for more than 10 days. Due to its physical properties and structure, the use of created biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising biomaterial for use in clinical practice.
The technique of obtainment of a new bioplastic material based on collagen, elastin and hyaluronic acid is described. The results of a study of the biomaterial structure and properties to assess the prospects for its further use in clinical practice are also presented. To prepare the material samples, collagen, elastin and hyaluronic acid were mixed in predetermined proportions with distilled water, and the resulting biofilm was crosslinked by UV irradiation. A commercial human skin fibroblast cell culture (HDF) was used to assess the biomaterial cytotoxicity and biocompatibility; as a result, it was shown that cytotoxicity is absent in it. The visualization and studies of the biomaterial structure were carried out using light microscopy. The new material was shown to be highly elastic and resilient; it also had a high porosity with a pore diameter of 100-200 um. It was shown that the biomaterial samples were able to maintain physical properties in the culture medium for more than 10 days, while the complete destruction of the matrix was observed 3-4 weeks after the beginning of incubation. Due to its physical properties and structure, and also the capacity of providing effective conditions for good cell proliferation, the created biomaterial can be used as a wound cover in the damaged skin areas. This allows us to consider the new biomaterial promising for clinical practice. cell technologies, tissue-engineered constructs, bioplastic material, collagen, human fibroblasts.
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