promising method for in vitro propagation of white sapota (Casimiroa edulis L.) was established. Shoot tips and nodal segments of C. eduils were cultured on Murashige and Skoog (MS) medium supplemented with 2.22 µM 6benzylamino purine (BAP) and 1.07µM ß-naphthalene acetic acid (NAA) recorded the maximum growth percentage of 88.88 and 77.77%, and average shoot length of 2.76 and 2.4 cm, for shoot tips and nodal segments, respectively. Regarding shoots multiplication rate, it was significantly affected by the concentration of BAP, as 6.77 shoots /explant were recorded using MS medium containing 13.32 µM BAP + 4.90 µM N6-2-isopenteny adenine (2iP). For rooting, half and full strength MS medium supplemented with 0.00, 2.46, 4.90, 9.80, 19.60 and 39.20 µM indol-3-butyric acid (IBA) in combination with 0.00 and 2.69 µM NAA were examined. The highest rooting percentage, average number of roots/shoot and average root length (44.44 %, 1.88 and 2.46 cm, respectively) were obtained on half strength MS medium containing 19.60 µM IBA and 2.69 µM NAA. Finally, plantlets were successfully acclimatized to greenhouse conditions and grew vigorously with no apparent phenotype aberrations.
Cupressus sempervirens L. has known medicinal activities, which are related to the presence of miscellaneous secondary metabolites, involved in patents for pharmaceuticals and/or cosmetics in the market. The aim of this study is to perform a comparative investigation of the main metabolites in the wild, cultivated and callus cultures of C. sempervirens using different treatments and the in vitro production of certain metabolites from C. sempervirens callus cultures in concentrations adequate for use commercially and also to develop a convenient chromatographic method for both the qualitative and quantitative determination of the most beneficial flavonoids (rutin and quercitrin), along with other metabolites (ferruginol, 2-furancarboxaldehyde,5 methyl, pentadecanoic acid, totarol, quinic acid and hinokiol) using HPLC/DAD and GC/MS, respectively. Leaf extract of the wild plant induced reduction in cell viability of some tested cell lines as: human hepatocellular carcinoma cell (HEPG2), lung carcinoma cell (A549), human Caucasian breast adenocarcinoma (MCF7) and especially on human colon cancer cell (HCT116), which was higher than doxorubicin used as a standard. This study is a stepping stone for obtaining natural and renewable bioactive secondary metabolites from C. sempervirens through in vitro callus cultures.
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