Given previous research on the risks associated with cycling in young adult dating relationships, the present study examines the frequency with which cyclical dating relationships (relationships that end and renew) persist into cohabitation and marriage, the characteristics of these relationships, and the constraints associated with cycling during these stages using a nationally representative sample of cohabiting (n = 323) and married (n = 752) couples. Using retrospective accounts, results suggest that over one-third of cohabiters and one-fifth of spouses have experienced a breakup and renewal in their current relationship. Additionally, partners who have experienced cycling are at greater risk for further cycling and experiencing greater constraints to permanently ending the relationship, greater uncertainty in their relationship’s future, and lower satisfaction.
DNA nanostructures (DNs) have been increasingly utilized in biosensing, drug delivery, diagnostics and therapeutics, because of their programmable assembly, control over size and shape, and ease of functionalization. However, the low cellular uptake of DNs has limited their effectiveness in these biomedical applications. Here we demonstrate the potential of membrane and glycocalyx binding as general strategies to enhance the cellular uptake of DNs. By targeting the plasma membrane and cell-surface glycocalyx, the uptake of all three distinct DNs is significantly enhanced as compared to uptake of bare DNs. We also demonstrate the viability of single-step membrane labeling by cholesterol-DNs as competitive with previous multistep approaches. Further, we show that the endocytic pathway of membrane-bound DNs is an interdependent process that involves scavenger receptors, clathrin-, and caveolin-mediated endocytosis. Our findings may potentially expand the toolbox for effective cellular delivery of DNA nanostructured systems.
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