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Normal values for intraocular pressure (IOP) and tear production in conscious cervids have not been reported to date. Based on trends in zoological institutions to perform non‐anaesthetized health exams, it is applicable to establish normal values in conscious animals, as anaesthesia and sedation can alter these parameters. The goal of this study was to estimate intraocular pressures using rebound tonometry and measure tear production values in a group of healthy, conscious, European fallow deer utilizing chute restraint. Evaluation of these values with regards to instrumentation and restraint variables will be assessed. Complete ophthalmic examinations, including estimation of IOP with rebound tonometry and measurement of tear production with Schirmer tear tests (STT) were performed on nine conscious European fallow deer (Dama dama) restrained in a chute. Correlations between IOP on the unspecified (P) and the equine (H) settings, as well as IOP and STT differences between left (OS) and right (OD) eyes were evaluated, in addition to assessment of correlations between right and left lateral recumbency on IOP and STT. Tear production measurements were 18.7 ± 5.1 mm min−1 with a 95% confidence interval (CI) range of 16.4–21.1 mm min−1. Intraocular pressure measurements for the P setting were 16.1 ± 4.5 mmHg with a 95% CI range of 14.1–18.2 mmHg, and for the H setting were 21.5 ± 5.1 mmHg with a 95% CI range of 19.1–23.9 mmHg. No statistically significant difference (P > 0.05) was found between OS and OD in any test. Neither left nor right lateral recumbency was found to have a statistically significant effect on IOP or STT. This study represents the first assessment of ophthalmic parameters in conscious fallow deer with rebound tonometry and STT.