Motion sickness (MS) is a syndrome associated with symptoms like nausea, dizziness, and other forms of physical discomfort. Automated vehicles (AVs) are potent at inducing MS because users are not adapted to this novel form of transportation, are provided with less information about the own vehicle’s trajectory, and are likely to engage in non-driving related tasks. Because individuals with an especially high MS susceptibility could be limited in their use of AVs, the demand for MS mitigation strategies is high. Passenger anticipation has been shown to have a modulating effect on symptoms, thus mitigating MS. To find an effective mitigation strategy, the prototype of a human–machine interface (HMI) that presents anticipatory ambient light cues for the AV’s next turn to the passenger was evaluated. In a realistic driving study with participants (N = 16) in an AV on a test track, an MS mitigation effect was evaluated based on the MS increase during the trial. An MS mitigation effect was found within a highly susceptible subsample through the presentation of anticipatory ambient light cues. The HMI prototype was proven to be effective regarding highly susceptible users. Future iterations could alleviate MS in field settings and improve the acceptance of AVs.