1. The flow regime is a primary determinant of the structure and function of aquatic and riparian ecosystems for streams and rivers. Hydrologic alteration has impaired riverine ecosystems on a global scale, and the pace and intensity of human development greatly exceeds the ability of scientists to assess the effects on a river-by-river basis. Current scientific understanding of hydrologic controls on riverine ecosystems and experience gained from individual river studies support development of environmental flow standards at the regional scale. 2. This paper presents a consensus view from a group of international scientists on a new framework for assessing environmental flow needs for many streams and rivers simultaneously to foster development and implementation of environmental flow standards at the regional scale. This framework, the ecological limits of hydrologic alteration (ELOHA), is a synthesis of a number of existing hydrologic techniques and environmental flow methods that are currently being used to various degrees and that can support comprehensive regional flow management. The flexible approach allows

Recognition of the escalating hydrological alteration of rivers on a global scale and resultant environmental degradation, has led to the establishment of the science of environmental flow assessment whereby the quantity and quality of water required for ecosystem conservation and resource protection are determined.A global review of the present status of environmental flow methodologies revealed the existence of some 207 individual methodologies, recorded for 44 countries within six world regions. These could be differentiated into hydrological, hydraulic rating, habitat simulation and holistic methodologies, with a further two categories representing combination-type and other approaches.Although historically, the United States has been at the forefront of the development and application of methodologies for prescribing environmental flows, using 37% of the global pool of techniques, parallel initiatives in other parts of the world have increasingly provided the impetus for significant advances in the field.Application of methodologies is typically at two or more levels. (1) Reconnaissance-level initiatives relying on hydrological methodologies are the largest group (30% of the global total), applied in all world regions. Commonly, a modified Tennant method or arbitrary low flow indices is adopted, but efforts to enhance the ecological relevance and transferability of techniques across different regions and river types are underway. (2) At more comprehensive scales of assessment, two avenues of application of methodologies exist. In developed countries of the northern hemisphere, particularly, the instream flow incremental methodology (IFIM) or other similarly structured approaches are used. As a group, these methodologies are the second most widely applied worldwide, with emphasis on complex, hydrodynamic habitat modelling. The establishment of holistic methodologies as 8% of the global total within a decade, marks an alternative route by which environmental flow assessment has advanced. Such methodologies, several of which are scenario-based, address the flow requirements of the entire riverine ecosystem, based on explicit links between changes in flow regime and the consequences for the biophysical environment. Recent advancements include the consideration of ecosystem-dependent livelihoods and a benchmarking process suitable for evaluating alternative water resource developments at basin scale, in relatively poorly known systems. Although centred in Australia and South Africa, holistic methodologies have stimulated considerable interest elsewhere. They may be especially appropriate in developing world regions, where environmental flow research is in its infancy and water allocations for ecosystems must, for the time being at least, be based on scant data, best professional judgement and risk assessment.

Despite their limited spatial extent, freshwater ecosystems host remarkable biodiversity, including one-third of all vertebrate species. This biodiversity is declining dramatically: Globally, wetlands are vanishing three times faster than forests, and freshwater vertebrate populations have fallen more than twice as steeply as terrestrial or marine populations. Threats to freshwater biodiversity are well documented but coordinated action to reverse the decline is lacking. We present an Emergency Recovery Plan to bend the curve of freshwater biodiversity loss. Priority actions include accelerating implementation of environmental flows; improving water quality; protecting and restoring critical habitats; managing the exploitation of freshwater ecosystem resources, especially species and riverine aggregates; preventing and controlling nonnative species invasions; and safeguarding and restoring river connectivity. We recommend adjustments to targets and indicators for the Convention on Biological Diversity and the Sustainable Development Goals and roles for national and international state and nonstate actors.

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