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A continuous culture apparatus was used to measure growth rates of Nitxschk cbsterium and T&w.seZmis sp. at different temperatures and light intensities and at two different levels of nitrate and phosphate cnrichmcnt of artificial senwntcr.A set of symmetrical relationships was found between light, temperature, and nutrient concentration in which the intcraction of any two of the factors was modified by varying the third. The optimum temperatures yielding maximum growth rates in a daily light-and-dark cycle were lower when a medium with nitrate and phosphate concentrations similar to those found in natural waters was used than when a medium having higher concentrations of these substances was used. The results of batch culture cxncriments with high nutrient concentrations may not bc applicable, thcrcfore, to natural situations.

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