Penelitian ini bertujuan untuk mengkaji parameter populasi ikan cakalang (Katsuwonus pelamis) yang tertangkap pukat cincin di WPP-572 Samudra Hindia Barat Sumatera dan WPP-573 Selatan Jawa. Hasil penelitian ini diharapkan dapat digunakan sebagai masukan dalam penentuan pengelolaan perikanan sehingga stok ikan cakalang dapat dimanfaatkan secara berkelanjutan. Ikan contoh dikumpulkan melalui program enumerasi tahun 2016 (Januari-Desember). Sampling acak dilaksanakan di empat pelabuhan, yaitu PPS Lampulo (Aceh), PPN Sibolga (Sumatra Utara), PPP Tamperan (Pacitan) dan IPP Pondokdadap (Malang). Jumlah ikan contoh diperoleh sebanyak 14.894 ekor. Serial data fekuensi panjang bulanan diolah menggunakan program FiSAT II untuk menduga parameter pertumbuhan, mortalitas dan tingkat eksploitasi. Hasil penelitian menunjukkan ikan cakalang yang tertangkap di WPP-572 memiliki panjang asimtotik (L)73,5 cmFL, K sebesar 0.22/tahun dan to sebesar -0,59 tahun. Parameter populasi di WPP-573 berturut-turut L”=67,20 cmFL, K=0,27/tahun, dan to=-0,50 tahun. Nilai mortalitas alami (M) ikan cakalang di WPP 572 sebesar 0,49/tahun, mortalitas total (Z) 0,70/tahun, dan kematian akibat penangkapan (F) adalah 0,21/tahun. Ikan cakalang yang tertangkap di WPP 573 menunjukkan nilai (E) sebesar 0,59/tahun, nilai Z 1,02/tahun, dan nilai F sebesar 0,43/tahun. Dugaan tingkat eksploitasi ikan cakalang di WPP 572 dan 573 masing-masing 0,3/tahun dan 0,42/tahun atau belum berada pada tahap optimal.This study aimed to assess the population parameters of skipjack tuna (Katsuwonus pelamis) that caught by purse seine in the Indonesian FMA (Fisheries Management Area) 572 Indian Ocean West Sumatera and FMA 573 South Java. The outcomes of this research can hopefully be used as inputs for fisheries management, in order the stock of skipjack tuna can be utilized sustainably. Fish sample were collected through the program enumeration of Research Institute for Tuna Fisheries (RITF) during the year 2016 (Januari-Desember). Using random sampling method at four fishing ports, namely PPS Lampulo (Aceh), PPN Sibolga (North Sumatra), PPP Tamperan (Pacitan), and IPP Pondokdadap (Malang). The total number of fish samples was 14.894 fish. Monthly length frequency data processed using FiSAT II program to estimate the growth parameters, mortality, and exploitation. The analysis results showed that skipjack tuna caught in FMA 572 has asymtotic length value (L) at 73.5 cmFL, K value 0.22/year, and to at -0.59 year; while in FMA 573 population parameters values respectively 67.20 cmFL, 0.27/year, and -0.50 year. The value of natural mortality (M) skipjack in FMA 572 is 0.49/year, total mortality (Z) 0.70/year, and fishing mortality (F) 0.21/year. Skipjack tuna that caught in FMA 573 showed value of M 0.59/year, Z value 1.02/year, and F value 0.43/year. The estimated values of exploitation levels of skipjack in FMA 572 and 573 were 0.3/year and 0.42/year respectively.
Skipjack tuna (Katsuwonus pelamis) is highly migratory species that spread from trophic and sub trophic waters. This species can be found in Atlantic, Indian and Pacific oceans. The genetic information of highly migratory species like skipjack tuna is important to support the sustainability of the fisheries. The objectives of this study are to gain information genetic diversity and population structure of exploited species and to understand the population kinship in Indonesian waters. Tissue samples were collected from six locations, i.e.: Sibolga (North Sumatera), Padang (West Sumatera), Binuangeun (Banten), Pacitan (East Java), Lombok (West Nusa Tenggara) and Kupang (East Nusa Tenggara). Microsatellite analysis was done in this study consisting of extraction, purification, polymerase chain reaction (PCR) amplification and electrophoresis. Three loci used for the analysis i.e.: UTD 172, UTD 523 and UTD 535. The results showed that there are two groups from six locations i.e.: group 1: Sibolga and Padang; group 2: Binuangeun, Pacitan Lombok and Kupang. The variance among these two groups is 0.066 with variance 5.441%. This finding in line with Indonesian Fisheries Management Area of 572 (west of Sumatera waters) and 573 (south of Java waters). However, as highly migratory species across nations, the management strategy for skipjack tuna needs collaboration among countries through regional fisheries management authority like Indian Ocean Tuna Commission (IOTC).
Ikan tongkol lisong dan krai merupakan salah satu jenis tuna yang berperan nyata untuk usaha perikanan tangkap di Indonesia. Pengelolaan sumberdaya ikan tersebut harus selalu dapat dilakukan untuk menjaga tingkat pemanfaatannya supaya tidak lebih tangkap. Kajian keragaman genetik merupakan salah satu teknik dalam pengelolaan pemanfaatan sumberdaya perikanan dengan cara mengetahui tingkat keragaman genetik pada suatu struktur populasi. Kajian keragaman genetik ini diharapkan dapat menjadi basis kajian stok dan opsi dalam pengelolaan sumberdaya perikanan tongkol agar pemanfaatannya dapat dilakukan secara berkelanjutan. Awal mula analisis keragaman genetik dilakukan dengan memperbanyak DNA secara in vitro menggunakan teknik PCR (Polymerase Chain Reaction). Keberhasilan proses PCR dipengaruhi oleh beberapa faktor seperti suhu dan waktu penempelan oligonukleotida primer. Berdasarkan hal tersebut, penelitian ini bertujuan untuk mengetahui suhu dan waktu optimal pada primer Aro2-38. Sampel penelitian diperoleh dari hasil tangkapan pukat cincin yang didaratkan di PPN Palabuhanratu, Jawa Barat. Optimasi PCR menggunakan 12 suhu dan 2 waktu penempelan yang berbeda yaitu : 520C; 52,80C; 540C; 55,50C; 57,20C; 59,10C; 60,90C; 62,80C; 64,50C; 65,90C; 67,20C dan 680C, dan suhu penempelan 30 dan 15 detik. Hasil analisis menunjukkan bahwa produk PCR optimum (menghasilkan pita alel DNA) pada ikan tongkol krai berhasil waktu penempelan 30 detik dengan rentang suhu 52-540C. Sedangkan pada sampel ikan tongkol lisong, produk PCR yang optimum muncul pada waktu penempelan 15 dan 30 detik, dengan rentang suhu 52-60,90C.Frigate and bullet tuna constitute one of tuna species that plays a significant role in Indonesian fishing business. Management of fisheries resources must always be done to maintain the level of utilization so that it is not excessive. Genetic study is one of techniques in managing fisheries resource utilization by knowing the level of genetic diversity in a population structure. This genetic diversity study is expected to be the basis and option in the management of tuna fishing resources so that their utilization can be carried out sustainably. Genetic diversity analysis is start by multiplying fish DNA using PCR (Polymerase Chain Reaction) technique. The success of the PCR process is influenced by several factors such as temperature and time of primary oligonucleotide attachment. Based on this, this study aims to determine the optimal temperature and time in primers Aro2-38. The research sample was obtained from the catch of purse seine landed in PPN Palabuhanratu, West Java. PCR optimization uses 12 temperatures and 2 different annealing times: 520C; 52.80C; 54ÚC; 55,50C; 57.20C; 59.10C; 60.90C; 62.80C; 64,50C; 65,90C; 67.20C and 680C, and the annealing times are 30 and 15 seconds. The results of the analysis showed that the optimum PCR product (producing DNA allele bands) on the cretaceous tuna was successfully pasted for 30 seconds with a temperature range of 52-540C. Whereas in the sample of tuna lisong, the optimum PCR product appeared at the time of attachment of 15 and 30 seconds, with a temperature range of 52-60.90C.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.