Twin arginine translocation (Tat) systems catalyze the transport of folded proteins across the bacterial cytosolic membrane or the chloroplast thylakoid membrane. In the Tat systems of Escherichia coli and many other species TatA-, TatB-, and TatClike proteins have been identified as essential translocase components. In contrast, the Bacillus subtilis phosphodiesterase PhoD-specific system consists only of a pair of TatA In bacteria, most of the exported proteins cross the cytosolic membrane through the Sec-dependent translocase in an unfolded conformation (1-3). A subset of proteins is transported via the twin arginine translocation (Tat) 2 pathway. This alternative route accepts folded proteins or protein domains as substrates (4 -6). Initially, the Tat pathway was discovered in chloroplasts as a pathway that operates independently of soluble factors and nucleoside triphosphates and is exclusively energized by the proton gradient across the membrane (7-9). In vitro translocation systems using Escherichia coli components demonstrated that also in bacteria this transport system is energized exclusively by the transmembrane proton electrochemical gradient (10,11). Substrates destined for export by the Tat system are synthesized as preproteins with a signal peptide containing an almost invariant twin arginine sequence motif in the N-region of the otherwise canonically structured signal sequence (12).In E. coli TatA, TatB, and TatC proteins have been demonstrated to be essential for Tat-dependent protein transport (13-16). The TatA homologous protein TatE has been proven to be functionally redundant (17). In chloroplasts structural and functional counterparts Tha4 (homologous to TatA; Ref. 18), Hcf106 (homologous to TatB; Refs. 16, 19), and cpTatC (homologous to TatC; Ref. 20) have been identified. The sequence-related proteins TatA and TatB are anchored in the cytoplasmic membrane via an amino-proximal ␣-helical domain (13). TatCs are a family of proteins with six calculated transmembrane-spanning domains with N and C termini exposed to the cytoplasmic or stromal side of the membrane (4). Gouffi et al. (21) recently proposed function-linked changes of TatA and TatC topologies for the mechanism of folded protein translocation in E. coli. These changes involve a TatA, the C terminus of which shuttles between the cytosol and the periplasmic space, and a TatC, the predicted fourth and fifth transmembrane helices of which shuttle in the periplasmic space. They speculated that this topology of TatC might reflect an operational state of TatC that changes during the protein translocation process (21). The current proposal for the action of Tat transport system of E. coli and plant thylakoids involves the initial binding of substrates to the TatB-TatC high molecular weight complex mediated via a direct contact of the double arginine signal peptide with TatC (20,22). The signal peptide binding triggers association with TatA to form the active translocation channel driven by the transmembrane proton electrochemical gradien...
Background: Kcnh1 is a voltage-gated potassium channel that is primarily expressed in brain.Results: Knockdown of kcnh1 in zebrafish delays neural development and causes embryonic lethality.Conclusion: Kcnh1 is involved in cell proliferation during early zebrafish development.Significance: The finding that Kcnh1 has basic functions beyond neural signaling will help to elucidate its roles in physiology and cancer formation.
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