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Soil-site characteristics and the biomass of trees larger than 5 cm dbh (diameter at breast height) were measured in a 50-year-old hardwood forest on a 269-ha watershed on the Allegheny Plateau. A weighted stepwise multiple regression model explained 65% of the variation in biomass. The significant variables were, in order of importance: aspect; iron and copper in the B horizon; lime requirement and calcium in the A horizon; calcium in the B horizon; surface stoniness; A horizon manganese, potassium, and iron.