Since 2015, the studies on in vitro rearing Braconidae and Trichogrammatidae (B. habetor Say, B. greeni and T. pintoe, T. evanescens, T. chilonis) have been conducted successfully in Uzbekistan. For these parasites, cooking and the three main types of media were made from the hemolymph of the wax moth (G. melonnella) and cotton worm Helicoverpa armigera (Heliothis armigera), egg yolk, natural milk 10%. The simulated "host-eggs" and "larvae" are made of polyethylene or polypropylene semi spherical capsules, containing artificial diets, in which insectan pupal hemolymph is the main component. Mass production of in vitro reared Braconidae and Trichogrammatidae its utilization in the fields showed good effectiveness in controlling cotton bollworm. In the article researches were given about creating artificial medium of diets in order to grow bracon and choosing effective medium of diets. In our researches medium of diets which consists of some components was created and they were harmed with bracon generations. According to it, generation of bracon’s nutrition and developing three main types of medium were produced in order to rear bracon parasite that was composed wax moths (G.melonnellan) and (heliothis armigera) hemolymph, egg yolk, natural milk.
In this article, species-compositions of pests found in velvet forest biocenosis have been identified, of which 48 species have seen more phytophages than others. In this, it turned out that the most affected forest trees are apples, pistachios, walnuts, almonds, Hawthorn, zhiyda. 23 species of parasites-entomophages belonging to 7 families have been identified that effectively control the number of these phytophages. During the studies, 1,180 butterflies, 6,510 eggs, 1,040 worms of different ages were collected and systematic analyzes were carried out in Forest agrobiosenosis from representatives of the genus tangachaganotli (Lepidoptera) from forest trees. 22 types of phytophages have been found to be more common than others. In this, it was known that the most affected forest trees are apples, pistachios, walnuts, almonds, Hawthorn, and nephews. In observations, it was known that in Forest agrobiosenosis, types of pests are more common than in other agricultural crops.
The article discusses the issue of mass reproduction in the biological laboratory of the ovarian parasite of Lepidoptera-Trichogramma, in particular the species Trichogramma evanescens Wes, widely represented in the nature of Uzbekistan. against the dangerous pest of cotton bollworm. In Uzbekistan, much attention is paid to the use of a biological method, in particular entomophages, which, unlike the chemical method, is characterized by safety for the environment and high biological efficiency reaching 69-75%. The article describes the technology of reproduction of trichogramma in a biological laboratory on eggs natural hosts of the entomophage.
Pomegranate (Punica granatum L.) is grown in tropical and subtropical regions of the world. Pomegranate has been grown in Central Asia for almost 2,000 years. More than 25% of pomegranates in Central Asia are located in Uzbekistan. Pomegranate fruit pest (Euzophera punicaella Mooze) is one of the main pests that reduce the basic quality and export yield of pomegranate. When chemical control measures are applied against them, pomegranates lose their quality and export character and do not always give the expected results. The larvae develop inside the fruit, which is why the effectiveness of medicines is low. We used entomophagous biological agents against this pest, such as Trichogram pintoi, Trichogram chilonis, Trichogram evanescens, Trichogram dendrolimi, and Trichogram ostrinae. For pomegranate fruit worms, Bracon hebetor Say entomophagy was highly effective when used in the laboratory in a ratio of 1:10. It is obvious that biological control measures provide high quality and high efficiency.
The issue of propagating parasitic insects in artificial food environments has been a challenge for researchers for a long time. Breeding one Trichogramma insect requires a lot of grain products and labor, and it takes about 40-50 days for Trichogramma and 55-60 days for Bracon to reproduce. This study aimed to develop a technology for the in vitro reproduction of trichogram, which are important parasitic entomophages in the control of various agricultural pests. The results of the experiments showed that the Trichogramma chilonis damage to nests with offspring was 82.4%, larvae emerged from eggs in 1.7 days. The larvae developed in this nutrient medium for 5.0 days, and the pupation period was 4.5 days. The average time from egg to imago was 11.2 days. The survival of hatchlings from the feeding medium lasted 5.4 days, and the male:female ratio (♂:♀) of hatched Trichograms was 1:6.
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