Background: Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent. Objective: To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes. Methods: Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 µM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated. Results: By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged. Conclusion: Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.
A B S T R A C T Background:The increasing use of herbal drugs and their ease of accessibility and availability have necessitated the use of mutagenicity test to analyze their toxicity and safety. Objectives: This study aimed to evaluate the genotoxicity of Sankol herbal medicine in DNA breakage of rat hepatocytes in comparison with H 2 O 2 by single cell gel electrophoresis technique or comet assay. Materials and Methods: In the current study hepatocytes were prepared from male wistar rats. Hepatocytes cells were counted and kept in a bioreactor for 30 minutes,then cells were exposed to Sankol herbal medicine at doses of 100, 200 and 400 µl/ml. Buffer 4 (incubation buffer) and H 2 O 2 were used for one hour as negative and positive control respectively. After 30 minutes cell suspension with low melting point agarose was put on precoated slides and covered with agarose gel. Then lysing, electrophoresis, neutralization and staining were carried out. Finally the slides were analyzed by fluorescence microscope. The parameter under this analysis was the type of migration which was determined according to Kobayashi pattern. Results: Results of the study indicated that by increasing the dose of Sankol herbal medicine, the DNA damage slightly increased (P < 0001). Conclusions:In overall compared to the positive control, significant differences were observed which indicated that the crude extract of Sankol in vitro did not have mutagenic effect.
A B S T R A C T Background:The increasing use of herbal drugs and their ease of accessibility and availability have necessitated the use of mutagenicity test to analyze their toxicity and safety. Objectives: This study aimed to evaluate the genotoxicity of Sankol herbal medicine in DNA breakage of rat hepatocytes in comparison with H 2 O 2 by single cell gel electrophoresis technique or comet assay. Materials and Methods: In the current study hepatocytes were prepared from male wistar rats. Hepatocytes cells were counted and kept in a bioreactor for 30 minutes,then cells were exposed to Sankol herbal medicine at doses of 100, 200 and 400 µl/ml. Buffer 4 (incubation buffer) and H 2 O 2 were used for one hour as negative and positive control respectively. After 30 minutes cell suspension with low melting point agarose was put on precoated slides and covered with agarose gel. Then lysing, electrophoresis, neutralization and staining were carried out. Finally the slides were analyzed by fluorescence microscope. The parameter under this analysis was the type of migration which was determined according to Kobayashi pattern. Results: Results of the study indicated that by increasing the dose of Sankol herbal medicine, the DNA damage slightly increased (P < 0001). Conclusions:In overall compared to the positive control, significant differences were observed which indicated that the crude extract of Sankol in vitro did not have mutagenic effect.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.