Purpose: this study was conducted between 2011 and 2017 at the faculty of Veterinary Medicine Cairo University and aimed to compare the efficiency of freshly isolated bone marrow (F-BMMSC) versus culture expanded bone marrow (E-BMMSC) when added to the mixture of autologous platelet rich fibrin (PRF), collagen with nanohydroxyapatite in healing of critical bony mandibular defects in mandibular site in dogs. Material and methods: 12 healthy adult Mongrel dogs were used in this study and bilateral mandibular critical bone defects (15X10 mm) were resected. Group A (The left side) contained: E-BMMSCs the expanded BMSC was seeded on collagen sponge with Nano-Hydroxyapatite and PRF membrane. Group B (The right side) contained: F-BMMSCs Gradient immediately separated BMSC seeded on the same scaffold. Radiographic follow up was done immediate, 4, 8, 12 weeks sacrificing was done 4, 8, 12 weeks postoperative. Local bone mineral densities (BMD) were measured on a DXA system. Histological evaluation was done using H&E. Results the results showed that group B right defects had better healing results than group A right defects all over the follow-up period, but the differences were not statically significant. Conclusion the study findings indicated that The F-BMMSCs that seeded in hydroxyapatite collagen type I HA-COL scaffolds combined with platelet rich fibrin PRF successfully repaired mandibular critical size defects, the same as the E-BMMSCs seeded in the same scaffold.
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