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Ocean acidification affects with special intensity Arctic ecosystems, being marine photosynthetic organisms a primary target, although the consequences of this process in the carbon fluxes of Arctic algae are still unknown. The alteration of the cellular carbon balance due to physiological acclimation to an increased CO 2 concentration (1300 ppm) in the common Arctic brown seaweeds Desmarestia aculeata and Alaria esculenta from Kongsfjorden (Svalbard) was analysed. Growth rate of D. aculeata was negatively affected by CO 2 enrichment, while A. esculenta was positively affected, as a result of a different reorganization of the cellular carbon budget in both species. Desmarestia aculeata showed increased respiration, enhanced accumulation of storage biomolecules and elevated release of dissolved organic carbon, whereas A. esculenta showed decreased respiration and lower accumulation of storage biomolecules. Gross photosynthesis (measured both as O 2 evolution and 14 C fixation) was not affected in any of them, suggesting that photosynthesis was already saturated at normal CO 2 conditions and did not participate in the acclimation response. However, electron transport rate changed in both species in opposite directions, indicating different energy requirements between treatments and species specificity. High CO 2 levels also affected the N-metabolism, and 13 C isotopic discrimination values from algal tissue pointed to a deactivation of carbon concentrating mechanisms. Since increased CO 2 has the potential to modify physiological mechanisms in different ways in the species studied, it is expected that this may lead to changes in the Arctic seaweed community, which may propagate to the rest of the food web.

In the fall, when 61% of the fronds of the Gelidium sesquipedale (Clem.) Born. et Thur. population located in Albufeira (southern Portugal) were reproductive, about 90% of these fronds were tetrasporophytes, whereas an equal percentage of female and male gametophytes was found (5%). The comparison of physiological performances of the reproductive phases (males, females and tetrasporophytes) did not reveal a physiological advantage of tetrasporic fronds. There were no significant differences either in the photosynthesis, nitrogen uptake, nitrate reductase activity, or biochemical composition of adult fronds. On the other hand, vegetative recruitment and spore production in the laboratory were significantly different. The re-attachment to calcareous substrate and the subsequent rhizoidal growth were faster in tetrasporophytes. Particular levels of temperature, rather than irradiance, had an important effect on the phase differences in the spore release, attachment, and germination rates. Significant results were the higher release of carpospores at all irradiances at 171 C, and the higher attachment percentage of carpospores at 131 C versus tetraspores. Under higher temperatures (211 C), tetraspores showed higher attachment rates while carpospores germinated more. G. sesquipedale cystocarps released carpospores for 2 months, while tetrasporangia stopped shedding tetraspores after 1 month, resulting in a 3-fold higher production of carpospores than tetraspores. Results showed that vegetative and spore recruitment may explain the low gametophyte-tetrasporophyte ratio of the studied population of G. sesquipedale as opposed to the physiological performance of phases.

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