Fluorescence microscopy images contain several channels, each indicating a marker staining the sample. Since many different marker combinations are utilized in practice, it has been challenging to apply deep learning based segmentation models, which expect a predefined channel combination for all training samples as well as at inference for future application. Recent work circumvents this problem using a modality attention approach to be effective across any possible marker combination. However, for combinations that do not exist in a labeled training dataset, one cannot have any estimation of potential segmentation quality if that combination is encountered during inference. Without this, not only one lacks quality assurance but one also does not know where to put any additional imaging and labeling effort. We herein propose a method to estimate segmentation quality on unlabeled images by (i) estimating both aleatoric and epistemic uncertainties of convolutional neural networks for image segmentation, and (ii) training a Random Forest model for the interpretation of uncertainty features via regression to their corresponding segmentation metrics. Additionally, we demonstrate that including these uncertainty measures during training can provide an improvement on segmentation performance.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.