Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD- or CMOS- camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold.

Biofilms – communities of microorganisms attached to surfaces – are a constant threat for long-term success in modern implantology. The application of laser scanning microscopy (LSM) has increased the knowledge about microscopic properties of biofilms, whereas a 3D imaging technique for the large scale visualization of bacterial growth and migration on curved and non-transparent surfaces is not realized so far.Towards this goal, we built a scanning laser optical tomography (SLOT) setup detecting scattered laser light to image biofilm on dental implant surfaces. SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells. Thus, the presented system allows the large scale investigation of vital biofilm structure and in vitro development on cylindrical and non-transparent objects without the need for fluorescent vital staining. We suggest SLOT to be a valuable tool for the structural and volumetric investigation of biofilm formation on implants with sizes up to several millimeters.

The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.

Correlative analysis requires examination of a specimen from macro to nano scale as well as applicability of analytical methods ranging from morphological to molecular. Accomplishing this with one and the same sample is laborious at best, due to deformation and biodegradation during measurements or intermediary preparation steps. Furthermore, data alignment using differing imaging techniques turns out to be a complex task, which considerably complicates the interconnection of results. We present correlative imaging of the accessory rat lung lobe by combining a modified Scanning Laser Optical Tomography (SLOT) setup with a specially developed sample preparation method (CRISTAL). CRISTAL is a resin-based embedding method that optically clears the specimen while allowing sectioning and preventing degradation. We applied and correlated SLOT with Multi Photon Microscopy, histological and immunofluorescence analysis as well as Transmission Electron Microscopy, all in the same sample. Thus, combining CRISTAL with SLOT enables the correlative utilization of a vast variety of imaging techniques.

Walking along a beach one may notice debris being washed ashore from the vast oceans. Then, turning your head up at night you even might noticed a shooting star or a bright spot passing by. Chances are, that you witnessed space debris, endangering future space flight in lower earth orbit. If it was possible to turn cm-sized debris into shooting stars the problem might be averted. Unfortunately, these fragments counting in the 100 thousands are not controllable. To possibly regain control we demonstrate how to exert forces on a free falling debris object from a distance by ablating material with a high energy ns-laser-system. Thrust effects did scale as expected from simulations and led to speed gains above 0.3 m/s per laser pulse in an evacuated micro-gravity environment.

Aiming for the generation of high-precision thrust in the µN range, focused high-intensity laser pulses are used employing the recoil of the jet of the ablated material. Whereas a single laser pulse yields an extremely low impulse bit in the range of several nNs, a broad thrust range can be accessed by the variation of the laser pulse repetition rate up to several hundreds of kilohertz. A detailed laser parameter study is carried out for aluminum and gold as propellant varying the pulse length from 100 fs to 10 ns and the fluence from 0.09 to 23.8 J/cm 2 . Two different regimes of thruster operation with respect to laser pulse length and specific impulse are identified. Irrespective of the pulse length regime, optimum impulse coupling is found at laser spot fluences around 2 J/cm 2 for aluminum and 20 J/cm 2 for gold, respectively, with coupling coefficients in the range of 25 to 40 µN/W. For ultrashort pulses, jet velocities are rather small yielding a specific impulse in the range of 70 s to 200 s, whereas for longer pulses beyond ≈ 100 ps, I sp is found to be in the range of 500 to 1000 s and beyond enabling low propellant consumption. However, ultrashort-pulse laser ablation might be favorable since material can be removed very smoothly which might contribute to very low thrust noise.

Thin-disk lasers are indispensable in photonics research as well as in a multitude of industrial applications. They represent a unique class of laser and amplifier architecture that provides kW output power with excellent properties concerning beam quality, long-term stability, thermal management, and power scalability. For many applications, a reduced complexity of the laser and its size would be highly beneficial. The necessary multipass transitions in thin-disk lasers and amplifiers typically require sophisticated multi-mirror arrangements. Here, we present a monolithic version of the pump concept for thin-disk lasers and amplifiers, where the thin disk is replaced by a thin, wedged gain medium acting as a wedged optical trap. The wedge is coated in a peculiar manner in order to allow for efficient in-and out-coupling of the pump and laser radiation from the wedge. This concept transfers the complexity of the multi-mirror optics into the thin disk itself in a monolithic fashion. With this concept, we achieved 890 W of CW output power, 59% slope efficiency, optical-to-optical efficiency of 50%, and a gain factor greater than 10 for small signals. This demonstrates that this new concept is capable of reaching the kW power regime with minimum complexity and size. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

The avoidance of any moving parts in a microthruster exhibits a great potential for low-noise thrust generation in the micronewton range. This is required, e.g., for scientific missions that need attitude and orbit control systems with exquisite precision. Laser ablation propulsion offers the opportunity of permanent inertiafree, electro-optical delivery of laser energy to access the propellant entirely without moving it. New propellant is accessed by ablating the previous surface in layers, essentially damaging the surface with a laser over and over again. The resulting surface properties for different fluences and scanning patterns were investigated for multiple layers of aluminum, copper, and gold. The pulse-length-specific issues of various ablation mechanisms such as vaporization, spallation, and phase explosion are accounted for by the use of a 10-ps laser system and a 500-ps laser system. We show that the surface roughness produced with 500-ps laser pulses is approximately twice the surface roughness generated by using 10-ps laser pulses. Furthermore, with 500-ps pulses, the surface roughness shows low dependency on the fluence for carefully chosen scanning parameters. Therefore, we conclude that laser pulse duration differences in the picosecond and nanosecond regimes will not necessarily alter surface roughness properties. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.