These data provide direct evidence of TF expression, activation of the extrinsic coagulation pathway, and thrombin formation in the surgical wound. Addition of pericardial blood to the perfusate and expression of TF by both circulating and adherent monocytes strongly promote thrombus formation during open heart surgery.
SummaryTo understand the functional changes induced by storage, we have examined the adenine nucleotides of platelets stored for 72 hr at 22°C and 4°C. Ten platelet concentrates (PC) were stored at each temperature with five in each group having a final volume of 50 ml and 30 ml. The total ATP and ADP content of platelets decreased following storage in all 4 groups of PC, with the decrease being greater in the PC stored at 22°C than those at 4°C. The mean thrombin secretable ATP+ADP content of platelets from PC stored at 22°C and 4°C were 29.7% and 19.7% of the total content, respectively (p <0.001). Thus, cold stored platelets have a higher total content of ATP+ADP but secrete distinctly lesser amounts than 22°C stored platelets. Labeling of the metabolic pool adenylates with 14C-adenine revealed a greater decrease in the adenylate energy charge of the platelets stored at 4°C. The secretion defect demonstrated in cold stored platelets may be related to the inability of these platelets to maintain ATP homeostasis.
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