Datura is a common plant in India that grows abundantly in the wild across the country. The common names of datura plant are Jimson weed, Stinkweed, Devil’s trumpet, Mad apple and Thorn apple etc. It is considered as a deliriant poison. Very few cases of intentional poisoning with Datura are reported in the literature. But accidental poisonings are though rare but not uncommon.Hereby we present a case of 37 years old male, who got poisoned by datura accidentally, following which he developed giddiness and was brought to our hospital. The diagnosis and treatment of the datura poisoning will be discussed.
We describe a safe and standardized perfusion protocol for studying brain pathology in high-risk autopsies using a custom-designed low-cost infection containment chamber and high-resolution histology. The output quality was studied using the histological data from the whole cerebellum and brain stem processed using a high-resolution cryohistology pipeline at 0.5 μm per pixel, in-plane resolution with serial sections at 20-μm thickness. To understand the pathophysiology of highly infectious diseases, it is necessary to have a safe and cost-effective method of performing high-risk autopsies and a standardized perfusion protocol for preparing high-quality tissues. Using the low-cost infection containment chamber, we detail the cranial autopsy protocol and ex situ perfusion-fixation of 4 highly infectious adult human brains. The digitized high-resolution histology images of the Nissl-stained series reveal that most of the sections were free of processing artifacts, such as fixation damage, freezing artifacts, and osmotic shock, at the macrocellular and microcellular level. The quality of our protocol was also tested with the highly sensitive immunohistochemistry staining for specific protein markers. Our protocol provides a safe and effective method in high-risk autopsies that allows for the evaluation of pathogen-host interaction, the underlying pathophysiology, and the extent of the infection across the whole brain at microscopic resolutions.
The World Health Organization has declared the outbreak of COVID-19 as a global pandemic. The alarming levels of spread and severity of the viral disease has resulted in significant morbidity and mortality. Women often face the highest risk of suffering devastating losses from the pandemic. Historically, women’s health has always been inadequately represented in responses to global outbreaks. Resources are often funnelled away from women’s health services towards targets perceived to be more important. Pregnant women with suspected, probable or confirmed COVID-19, should have access to obstetric and foetal medicine, neonatal care as well as mental health and psychosocial support, at facilities ready to tackle maternal and neonatal complications. In this article, we attempt to look at the challenges faced by gynaecologists because of this pandemic, and provide an overview on the current protocols in antenatal care, foetal care, childbirth, and oncological care.
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