Otitis media (OM) is a worldwide major health and economic issue leading to hearing loss, especially in developing countries due to high antibiotic resistance among the causative pathogens. This study aims to isolate, purify and determine the prevalence of MDR microorganisms causing OM. This is in addition to estimate in vitro effectiveness of irradiated and un-irradiated aqueous garlic extract (AGE). Finally, the present study aims to evaluate the enhancement of CIP, TOB, and NYS, commonly used in OM treatment, with IAGE against identified MDR bacteria and fungi. In the current investigation, the in vitro data revealed that OM is more prevalent in middle aged adults than in children. OM were predominated by bacterial isolates (59.0%), followed by fungal isolates (41.0%), including Pseudomonas aeruginosa (5 isolates), Proteus mirabilis (3 isolates), in addition to Klebsiella pneumoniae, Alcaligenes faecalis, Enterococcus faecalis, Bacillus cereus, Penicillium chrysogenum, Aspergillus flavus, and Aspergillus niger (1 isolate). Irradiated AGE (IAGE) showed nearly the same antimicrobial activity as the un-irradiated AGE. The inhibition activity enhancement was more statistically significant (P< 0.001) on combination of IAGE with CIP\NYS than with TOB (P< 0.05) and (P< 0.001) compared with each alone. Noticeable morphological changes in P. aeruginosa and A. flavus were observed by TEM images after the combination of IAGE with CIP/NYS. This study is one of primarily attempts to evaluate the IAGE to be used in future as a natural, safe, sterile, low cost, and available whole additive in therapeutic protocols to overcome MDR problem
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.