Orchidaceae adalah nama latin dari bunga anggrek yang memiliki berbagai jenis bunga sehingga memiliki ciri-ciri yang berbeda antara satu jenis dengan jenis yang lain, mulai dari ciri warna, bentuk, maupun ukuran. Hampir di seluruh daerah di Indonesia memiliki jenis tanaman anggrek tersendiri sesuai dengan karakter geografis daerah tersebut. Untuk membedakan jenis tanaman anggrek maka bisa dilihat dari kondisi tekstur atau warna dari kelopak bunga anggrek. Namun karena kemiripan ciri tekstur atau warna dari kelopak bunganya, maka kegiatan identifikasi jenis tanaman anggrek oleh beberapa orang atau petugas terkait masih cenderung subjektif sehingga menimbulkan kesalahan dalam kegiatan identifikasi. Penelitian ini bertujuan untuk membantu identifikasi jenis tanaman anggrek menggunakan teknologi pengolahan citra digital sehingga memperoleh hasil yang akurat. Dalam penelitian ini jenis tanaman anggrek yang diteliti meliputi jenis dendrobium, phalaenopsis dan vanda. Tahapan yang dilakukan dalam penelitian adalah mengekstraksi tekstur bunga anggrek menggunakan metode tapis gabor, sedangkan untuk klasifikasi menggunakan metode M-SVM (Multiclass Support Vector Machine). Hasil dari penelitian ini memiliki akurasi klasifikasi mencapai 95,4%.
The objective of this research was to determine the effect of the simple modified freezing method, 1°C/minute freezing rate with different diluter ration on a postthawing quality of local goat sperm namely Peranakan Etawah (PE). This work is aimed to study the quality of post-thawing sperm and to characterize the calcium intensity profile of both fresh and post thawing goat sperm. The method used is the experimental design of a laboratory. Freezing semen was performed in 2 main temperatures of-45°C then-196°C respectively using Mr. Frosty (®) System. Early Sperm characters of Ca +2 intensity was performed by Confocal Laser Scanning Microscope (CLSM) through Fluo-3 staining and Ca ++ intensity was analysis descriptively. The result showed that postthawing qualities are considered as good as standard qualities, at least, more than 40% based on Indonesian National Standard (SNI, 2014). The different level diluents commercial of Andromeda used were influenced highly significant (P<0.01). The best diluents ration is 1:4 (v/v) for final sperms stocked at-196°C. However freezing sperm conserved in-196°C is better than in-45°C. Meanwhile, the sperm characters of two condition showed the important variation of Ca +2 intensity, with the length of region measurement of 39.06±4.595 and 32.696±9.011 µm each. It was concluded that the calcium intensity pattern was varied more and higher in fresh sperm than in freezing sperms. This simple modified method of a freezing system was considered as a feasible alternative method for goat semen in a reason for both for sperm post-thawing quality and practical purposes.
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