BackgroundThis study investigated the prevalence of Enterococcus faecalis, its putative virulence factors and antimicrobial susceptibility in individuals with and without dental diseases. A total of 159 oral rinse specimens were collected from patients (n = 109) suffering from dental diseases and healthy controls (n = 50).ResultsE. faecalis was detected using only culture in 8/109 (7.3%) of the patients with various types of dental diseases, whereas no E. faecalis was found in the healthy controls weather using both culture and PCR. Phenotype characterizations of the 8 E. faecalis isolates indicated that 25% of the isolates produced haemolysin and 37.5% produced gelatinase. Most important virulence genes; collagen binding protein (ace) and endocarditis antigen (efaA) were present in all 8 E. faecalis isolates, while haemolysin activator gene (cylA) was detected only in 25% of isolates, and all isolates were negative for esp gene. All E. faecalis isolates were 100% susceptible to ampicillin, chloramphenicol, ciprofloxacin, vancomycin, and teicoplanin, and to less extent to erythromycin (62.5%).ConclusionThis study shows that all E. faecalis isolates were recovered only from patients with dental diseases especially necrotic pulps, and all isolates carried both collagen binding protein and endocarditis antigen genes and highly susceptible to frequently used antimicrobial drugs in Jordan.
In the recent years, emergence of infectious diseases caused by drug resistant pathogens had been increased; therefore there is an urgent need to search for new alternative and effective antimicrobial agents to overcome the drug resistance. In the present investigation, the study group consisted of 90 sheep and 90 goats with clinical evidences of mastitis in 17(18.89%) goats and 5 sheep (5.56%) that manifested swollen udder with or without systemic signs of illness related to mastitis. Standard bacteriology was performed on pretreatment milk samples from the 17 goats and 5 mastitic ewes as well as 60 soil samples and 60 pail water samples. The bacteria isolated were identified as Staphylococcus aureus 12 (70.6%) from goats and 5 (100%) from sheep. In addition, S. aureus could also be identified in 41 (68.3%) soil samples and 42 (70%) water samples. The current study aimed to explore the bactericidal effect of silver nanoparticles (AgNPs) on methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) strains isolated from mastitic milk and their surrounding environment (water and soil) in Aswan, Egypt. AgNPs solution was synthesized by typical one-step synthesis protocol using soluble starch and was characterized using transmission electron microscopy and atomic absorption spectrophotometer. The minimum inhibitory concentration (MIC) and minimum Article history:
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