More than 50% of the world’s population consumes rice. Accurate and up-to-date information on rice field extent is important to help manage food and water security. Currently, field surveys or MODIS satellite data are used to estimate rice growing areas. This study presents a cost-effective methodology for near-real-time mapping and monitoring of rice growth extent and cropping patterns over a large area. This novel method produces high-resolution monthly maps (10 m resolution) of rice growing areas, as well as rice growth stages. The method integrates temporal Sentinel-1 data and rice phenological parameters with the Google Earth Engine (GEE) cloud-based platform. It uses monthly median time series of Sentinel-1 at VH polarization from September 2016 to October 2018. The two study areas are the northern region of West Java, Indonesia (0.75 million ha), and the Kedah and Perlis states in Malaysia (over 1 million ha). K-means clustering, hierarchical cluster analysis (HCA), and a visual interpretation of VH polarization time series profiles are used to generate rice extent, cropping patterns, and spatiotemporal distribution of growth stages. To automate the process, four supervised classification methods (support vector machine (SVM), artificial neural networks (ANN), random forests, and C5.0 classification models) were independently trialled to identify cluster labels. The results from each classification method were compared. The method can also forecast rice extent for up to two months. The VH polarization data can identify four growth stages of rice—T&P: tillage and planting (30 days); V: vegetative-1 and 2 (60 days); R: reproductive (30 days); M: maturity (30 days). Compared to field survey data, this method measures overall rice extent with an accuracy of 96.5% and a kappa coefficient of 0.92. SVM and ANN show better performance than random forest and C5.0 models. This simple and robust method could be rolled out across Southeast Asia, and could be used as an alternative to time-consuming, expensive field surveys.

Ascochyta rabiei is the causal organism of ascochyta blight of chickpea and is present in chickpea crops worldwide. Here we report the release of a high-quality PacBio genome assembly for the Australian A. rabiei isolate ArME14. We compare the ArME14 genome assembly with an Illumina assembly for Indian A. rabiei isolate, ArD2. The ArME14 assembly has gapless sequences for nine chromosomes with telomere sequences at both ends and 13 large contig sequences that extend to one telomere. The total length of the ArME14 assembly was 40,927,385 bp, which was 6.26 Mb longer than the ArD2 assembly. Division of the genome by OcculterCut into GC-balanced and AT-dominant segments reveals 21% of the genome contains gene-sparse, AT-rich isochores. Transposable elements and repetitive DNA sequences in the ArME14 assembly made up 15% of the genome. A total of 11,257 protein-coding genes were predicted compared with 10,596 for ArD2. Many of the predicted genes missing from the ArD2 assembly were in genomic regions adjacent to AT-rich sequence. We compared the complement of predicted transcription factors and secreted proteins for the two A. rabiei genome assemblies and found that the isolates contain almost the same set of proteins. The small number of differences could represent real differences in the gene complement between isolates or possibly result from the different sequencing methods used. Prediction pipelines were applied for carbohydrate-active enzymes, secondary metabolite clusters and putative protein effectors. We predict that ArME14 contains between 450 and 650 CAZymes, 39 putative protein effectors and 26 secondary metabolite clusters.

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This article documents the addition of 139 microsatellite marker loci and 90 pairs of singlenucleotide polymorphism sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Aglaoctenus lagotis, Costus pulverulentus, Costus scaber, Culex pipiens, Dascyllus marginatus, Lupinus nanus Benth, Phloeomyzus passerini, Podarcis muralis, Rhododendron rubropilosum Hayata var. taiwanalpinum and Zoarces viviparus. These loci were cross-tested on the following species: Culex quinquefasciatus, Rhododendron pseudochrysanthum Hay. ssp. morii (Hay.) Yamazaki and R. pseudochrysanthum Hayata. This article also documents the addition of 48 sequencing primer pairs and 90 allele-specific primers for Engraulis encrasicolus. et al.

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