Post-harvest deterioration is the most important cause of loss in cassava production and this is mainly as a result of microbial invasion of the tubers. This research was therefore carried out to identify and control the organisms responsible for post-harvest deterioration of cassava tubers. Ethanolic and water extractions ofAzadirachta Indica(A. Juss) leaves andAframomum melegueta(Schumann) seeds were used as antifungal agents and the susceptibility of four of the isolated pathogenic fungi to them was observed in culture. The tested organisms wereAspergillus nigerVan Tiegh,Botryodiplodia theobromaePat,Fusarium solani(Mart). Sacc andPenicillium oxalicumCurrie and Thom. All tested organisms showed different degrees of inhibitions by the extracts withA. nigerbeing the most inhibited by ethanolic extraction ofA. melegueta. The overall result showed thatA. indicawas more active on the organisms though it’s effects and that ofA. meleguetawere not significantly different (P>0.05).
a peer-reviewed online international journal allowing free unlimited access to abstract and full-text of published articles. The journal is devoted to the promotion of health sciences and related disciplines (including medicine, pharmacy, nursing, biotechnology, cell and molecular biology, and related engineering fields). It seeks particularly (but not exclusively) to encourage multidisciplinary research and collaboration among scientists, the industry and the healthcare professionals. It will also provide an international forum for the communication and evaluation of data, methods and findings in health sciences and related disciplines. The journal welcomes original research papers, reviews and case reports on current topics of special interest and relevance. All manuscripts will be subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication) will be published without delay. The maximum length of manuscripts should normally be 10,000 words (20 single-spaced typewritten pages) for review, 6,000 words for research articles, 3,000 for technical notes, commentaries and short communications. Submission of Manuscript:The International Journal of Health Research uses a journal management software to allow authors track the changes to their submission. All manuscripts must be in MS Word and in English and should be submitted online at http://www.ijhr.org. Authors who do not want to submit online or cannot submit online should send their manuscript by e-mail attachment (in single file) to the editorial office below. Submission of a manuscript is an indication that the content has not been published or under consideration for publication elsewhere. Authors may submit the names of expert reviewers or those they do not want to review their papers. AbstractThis review discusses the conceptual aspects of method validation, its management, processes and schemes and highlights method validation key performance characteristics. Although a thorough validation cannot rule out all potential problems, the process of method development and validation should address the most common ones. Examples of typical problems that can be minimized or avoided are synthesis impurities that co-elute with the analyte peak in an HPLC assay; a particular type of column that no longer produces the separation needed because the supplier of the column has changed the manufacturing process; an assay method that is transferred to a second laboratory where they are unable to achieve the same detection limit; and a quality assurance audit of a validation report that finds no documentation on how the method was performed during the validation. Problems increase as additional people, laboratories, and equipment are used to perform the method. When the method is used in the developer's laboratory, a small adjustment can usually be made to make the method work, but the flexibility to change it is lost once the method is transferred to other laboratories or used for official product testing. This is...
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