Studies of our experiment are related to the binding of enzymes to antibodies. This involves the formation of a stable, covalent linkage between an enzyme & antibody. We used different Enzymes for this studies which are altered in binding action. Enzymes we used-e.g. Horseradish peroxidase (HRPO), urease, or alkaline phosphatase. Studies of Enzyme and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site nor the active site of the enzyme is functionally altered. The enzyme most commonly used in the immunoreagent (the antibody enzyme conjugate) preparation is horseradish peroxides. This enzyme is cheap and can be attached to the immunoreagent by a variety of methods. Moreover many chromogenic substrates for it are also available. Our Experiment studies on conjugation of Horseradish Peroxidase (HRPO) to antibody (anti human IgG) will be carried out by Periodate oxidation method. We have prepared Anti human IgG-HRP conjugate in our laboratory. This will be used for ELISA and western blot experiments and IgG purified from different human serum samples would be used as the antigen in the above experiments. This experiment is carried out in two different steps mentioned as: Preparations of anti-human IgG-Horseradish peroxidase conjugate, Characterization of isolated human IgG.
Mosquitoes include the major vector population for the transmission of many diseases for global mortality and morbidity with increased resistance to common insecticides. Aedes aegypti vector of dengue is spread into many parts of the globe, a study intend to investigate the efficacy of the leaf extract of Morinda tinctoria and silver nanoparticles (AgNps) synthesized using M. tinctoria against the third instar larvae of Ae. aegypti. AgNps were synthesized from leaf extract of M. tinctoria and its effects against 3 rd instar larvae of Ae. aegypti were evaluated in the laboratory. The produced nanoparticles were subjected to different analysis include UV-Vis spectroscopy, Atomic Force Microscopy (AFM) and Fourier Transform Infrared Radiation (FTIR) spectroscopy. Both The leaf extract and the synthesized AgNps were tested against the 3 rd instar larvae of Ae. aegypti and the recorded 50% lethal concentration (LC 50) were 11.716 ppm and 3.631 ppm respectively. The results recorded from UV-Vis spectroscopy, AFM and FTIR Spectroscopy support the biosynthesis and characterization of AgNps. The results suggested that the leaf extract of M. tinctoria and synthesis of AgNps have the potential to be used as an ideal eco-friendly approach towards the control of Ae. aegypti in the field.
The declaration of COVID-19 as a pandemic disease by WHO has led to drastic changes in the healthcare environment within a short span. These changes were also dynamic requesting an efficient leadership skill to adopt and implement the changes demanded. This system study was carried out in a Cardio-Thoracic tertiary care hospital treating COVID patients, to identify the problem faced during various phases and their good practices along with their adherence to the National & International guidelines. It is a descriptive observational study carried in the hospital along with document study and interview of the staff involved in COVID management. This study concludes that an effective leadership with structured organogram in a healthcare setup during a disaster like COVID-19 pandemic could drastically improve the healthcare management both clinically and administratively, provided the resources are optimally used and the workforce strictly adhering to the hospital policies and guidelines.
Lipoma is the benign tumor of adipose tissue. It constitutes about 1 to 2% of all benign neoplasms of the oral cavity. Intraorally, it occurs predominantly in the buccal mucosa followed by tongue, floor of the mouth, buccal vestibule and rarely in the palate and gingiva. An 80-year-old male reported with a swelling in the right buccal mucosa for the past two years. Clinically the swelling was light yellow in color and had a broad peduncle. The swelling was soft in consistency and mobile. A provisional diagnosis of a lipoma was made. The tumor was excised and confirmed by histopathological examination.
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