The detection of harmful chemicals and biological agents in real time is a critical need for protecting water quality. We studied t he real -time eff ects of fiv e env ironmental c ontaminants with di ffering modes o f act ion (atrazi ne, pentachlorophenol, cadmium chloride, malathion, and potassium cyanide) on respiratory oxygen consumption in 2-day post-fertilization f athead m innow (Pimephales promelas) eg gs. Ou r o bjective was to a ssess t he se nsitivity o f f athead minnow eggs using the self-referencing micro-optrode technique to detect instantaneous changes in oxygen consumption after brief exposures to lo w c oncentrations of contaminants. Oxy gen co nsumption data i ndicated that the technique is indeed sensitive enough to reliably detect physiological alterations induced by all co ntaminants. After 2 h of exposure, we i dentified significant increases i n o xygen consumption u pon e xposure t o pen tachlorophenol (100 an d 1000 µ g/L), cadmium chloride (0.0002 and 0.002 µg/L), and atrazine (150 µg/L). In contrast, we observed a significant decrease in oxygen flux af ter e xposures to pot assium c yanide (5.2, 22, an d 44 µ g/L) a nd at razine ( 1500 µ g/L). N o ef fects were detected af ter e xposures t o malathion (200 an d 340 µ g/L). We h ave al so t ested t he s ensitivity o f Daphnia magna embryos as a nother a nimal model for real -time e nvironmental bio monitoring. Ou r res ults are s o f ar en couraging and support fu rther dev elopment of t his t echnology as a p hysiologically co upled bi omonitoring tool f or t he det ection o f environmental toxicants.
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