Various studies have been conducted to see the scaffold that supports the regeneration of tendon. This study aims to analyze the in vitro secretome tenogenic potential produced by ASCs culture with fresh frozen tendon scaffold in hypoxic conditions. ELISA tests for Scx and IGF-1 levels in secretome were obtained from ASC culture with fresh frozen tendon scaffold under normoxic (21%) and hypoxia (2%) conditions. The immunohistochemical examination of COL-1 was also carried out on the 2nd and 6th days of cell culture. The secretion of Scx and IGF-1 was increased in secretome from ASC cultures using a fresh frozen tendon scaffold compared with those which did not (p <0.05). In the normoxia condition, Scx and IGF-1 in secretome with fresh frozen tendons had better results than hypoxic conditions (p <0.05). The highest Scx levels were obtained in culture on the 6th day (p <0.05), while the highest IGF-1 levels were obtained in the culture on the 2nd day (p <0.05). There was an increase in the secretion of Scx and IGF-1 from ASC cultures with fresh frozen tendon scaffold under the hypoxic condition of 2%.
Introduction: Meniscus root tears are defined as radial tears within 1 cm of the meniscus insertion, or avulsions at the insertion of the meniscus. Based on tear morphology, there are 2 classification system for both medial and lateral posterior root tears. Lateral meniscus posterior root tear (LMPRT) is usually traumatic in nature and has been reported to occur in 7-10% of patients with anterior cruciate ligament (ACL) injury. The most commonly utilized treatments for LMPRT include non-operative treatment, partial meniscectomy, or repair. However, studies evaluating the clinical success of partial meniscectomy for LMPRT are contradictory. Case Report: A 18 years old man came to the orthopaedic clinic with complaints of pain in the right knee, after 2 months excessive physical training to join the police academy. Physical examination of the right knee showed positive results on McMurray and Thesaly’s test. According the combination of the cleft, truncated triangle, and ghost signs on the patient’s MRI examination which showed there was a lateral meniscus posterior root tear of the right knee without ACL injury. Meniscus repair was performed for this patient at the beginning, but apparently the meniscus can not be repositioned. Therefore, we decided that Arthroscopy Partial Meniscectomy (APM) was the best option for this patient. Conclusion: Even though APM long term outcomes is still controversial, when repair is not possible, APM is preferred to get short term benefits after the surgery, such as shorter recovery time and less revision surgery rates.
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