An experimental study has been conducted to determine the effects of extraction time and pH variation on the yield of pectin isolation from pomace of peento peaches. Ethanol volume (EV) and pectin solution (extract) evaporation were defined to investigate their influence on the precipitation pH and the yield of pectic substances extraction. The yield rose as a result of the increase in initial pH, time of extraction and EV at pH less than 3.5. In contrast, further increase in pH resulted in a drop in the yield. The maximum yield of pectin extraction, 7.03% ± 0.2%, was obtained at initial pH 2.5, EV of 1.5 and acid washing time of 120 min. In addition, the effect of EV on the degree of esterification (DE) and ash content of extracted pectic substances was determined by FTIR and ashing methods, respectively. Results indicated that EV can affect DE strongly.
Secondary structural changes and partitioning process of bovine serum albumin (BSA) in the mixture of (poly-(ethylene glycol 2000) + K 2 HPO 4 + H 2 O) have been studied at 23°C. The effect of varying weight percents of poly(ethylene glycol) and K 2 HPO 4 on the secondary structure of partitioned BSA and the partitioning process of BSA were examined. The equilibrium weight percents of the obtained aqueous two-phase system (ATPS) have been evaluated, and the phase diagrams including the tie lines are presented. It was found that the magnitude of the partition coefficient of BSA and the secondary structures of partitioned BSA depend on the length of obtained tie lines. Occurrences of changes on the secondary structure content of the partitioned BSA were observed by FTIR spectroscopy investigation. The amounts of the secondary structure content of partitioned BSA as R-helix, -sheet, -turn, and unordered structures of BSA were determined and reported.
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