Consequence of dietary fish meal substitution with wheatgrass was evaluated by observing growth response, associated feed cost and survival of grass carp (Ctenopharyngodonidella) fingerlings for sixty days. Sprouted wheatgrass (Triticumaestivum) was prepared for its inexpensively rich nutrients. Four isonitrogenous test diets were formulated and applied as treatments (T) in triplicates (R). In the control (T1), basal inclusion rate of fish meal was 30%, of which 10% was replaced with wheatgrass powder in T2 and in T3 replacement was 20%. In contrast, 30% of basal fishmeal was replaced in T4. Grass carp fingerlings (6.38±0.21 cm and 2.83±0.36 g) were stocked in twelve aquaria (60×40×45 cm³) each containing 75 L water, at 10 fish per aquarium, fed test diets at 5% of body weight twice daily. Prominent effect of wheatgrass supplementation was found on food conversion ratio (FCR) and survival rates. The significantly lowest FCR was observed in T3 (2.13±0.42) followed by T2 (2.89±0.99), T1 (3.01±1.53) and T4 (3.05±0.94). Besides, fish survival rate was significantly improved in T2 (90%), T3 (93.33%) and T4 (93.33%) compared to the lowest survival in T1 (83.33%). In conformity, fish tolerance (LT50) to low pH stressor was also increased with wheatgrass supplementation. The other growth parameters among the treatments were statistically similar with highest specific growth rate and fish production in T3 (1.13±0.12 %/day and 2.28±0.13 tons/ha). Dietary wheatgrass did not affect the fish carcass composition rather gave better result to some extents. The significantly highest carcass protein and lowest moisture was retained in T3 (14.13±0.05% and 74.91±0.25% respectively), whereas comparatively higher lipid and mineral (ash) content was found in T1 (7.69±0.02% and 2.35±0.27% respectively). Importantly, feed formulation cost was reduced by 2.61, 4.89, and 7.71% in T2, T3, and T4 respectively compared to T1. Therefore, wheatgrass could be promising in juvenile grass carp diet. Asian J. Med. Biol. Res. September 2020, 6(3): 482-490
Wheatgrass was evaluated as a potential non-conventional feedstuff to supplement fish meal in juvenile rohu (Labeorohita) diet to reduce feed cost. Green leafy sprouted wheatgrass (Triticumaestivum), inexpensive quality nutrient source, was processed into powder to formulate sinking pellet feed. Four isonitrogenous test diets were applied in four treatments (T) with three replications (R) each. The basal inclusion rate of fish meal was 30% in the control (T0), of which 10, 20 and 30% was replaced with wheatgrass powder in T10, T20 and T30 respectively to feed the experimental fish. Rohu fingerlings (7.63±0.41 cm; 4.66±0.15 g) were stocked in twelve glass aquaria (60×40×45 cm³) at 10 fish in 75 L water per aquarium, fed experimental diets at 5% of body weight twice daily. After 60 days of feeding trial, significantly better growth was observed in T20 with the highest production (3.23±0.44 tons/ha/60days), SGR (1.01±0.08 %/day) and the lowest FCR (2.68±1.40). Importantly, fish survival rate was improved with the progressive addition of wheatgrass in T10 (90%), T20 (93.33%) and T30 (100%) compared to the lowest survival in T0 (86.67%). Correspondingly fish in T30 were most resilient to low pH stress test (LT50 = 17 minutes) followed by T20 and T10 than T0 (LT50 = 9 minutes). Supplementation also resulted in better fish carcass quality with lowest carcass lipid (3.96±0.15%) and highest protein (15.72±0.53%) in T20 feed cost was reduced by 2.73, 5.13 and 8.06% in T10, T20 and T30 respectively than T0. Therefore, wheatgrass has prospect in juvenile rohu diet. Res. Agric., Livest. Fish.7(3): 533-543, December 2020
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