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A real-time PCR (RT-PCR) assay was designed for the simultaneous identification of and its ciprofloxacin susceptibility status. A SYBR green-based multiplex RT-PCR format was used; it comprised two different forward primers and a common reverse primer to detect single nucleotide polymorphisms (SNPs) in of The primer pairs were evaluated for their sensitivity and specificity using genomic DNA from 254 isolates (82 were ciprofloxacin susceptible and 172 were ciprofloxacin resistant) and 23 non- species isolates. The performance of the primers was validated using genomic DNA from 100 different isolates (46 were ciprofloxacin susceptible and 54 were ciprofloxacin resistant) and 52 non- isolates. The latter panel was revalidated by testing 99 (46 isolates were ciprofloxacin susceptible and 53 isolates were ciprofloxacin resistant) of the isolates and 23 non- isolates. These primers detected and its ciprofloxacin susceptibility status with over 99% sensitivity and specificity for all panels tested. This assay has the potential to be an inexpensive and rapid test for the simultaneous identification of and its ciprofloxacin susceptibility status.