a b s t r a c tDecolorization of azo dye, Eriochrome Black T was studied using the bacterium, Bacillus cereus RC1, isolated from dye contaminated site. At an initial dye concentration of 100 mg/L, the decolorization efficacy of 78.15% was observed in 24 h at pH 7. The effect of process and medium parameters such as pH, temperature, dye concentration, glucose, yeast extract and (NH 4 ) 2 SO 4 on the decolorization of dye by the bacterium was evaluated using response surface methodology (RSM) involving a central composite design. The optimum conditions determined for the decolorization by RSM were pH 7.01, temperature 35.39°C, initial dye concentration 106.25 mg/L, glucose 3.06 g/L, yeast extract 0.75 g/L and (NH 4 ) 2 SO 4 1.11 g/L. Under the optimized conditions, the decolorization of dye was found to be 92.34% which was in good agreement with the predicted value of 93.47% in 24 h of incubation. The nontoxic nature of the decolorized end product was determined by phytotoxicity study using tomato seeds. Hence, the bacterial isolate can be employed in dye decolorization process in industrial wastewater treatment as an eco-friendly and nontoxic alternative.
Citation: Rajeswari Uppala, Krishnan Sundar and Azhaguchamy Muthukumaran, Decolorization of Azo Dyes using Dried Biomass of B. cereus RC1 and Kocuria kristinae RC3, J Pure Appl Microbiol., 2019; 13(4):
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