DNA of live cells is damaged when exposed to harmful Ultraviolet rays. If the cells are exposed to UV rays exposure time more duration of time, more thymine dimers are formed in the DNA and the greater the risk of an incorrect repair or a missed dimer. To analyze the effect of UV rays Calf thymus DNA and prevention by polyphenol enriched extract of Mint leaves and also its non toxic nature. The above study was done by using submarine gel electrophoresis where UV rays are used to damage the DNA and BHA (400µM) used as positive control. The polyphenol enriched extract was taken at 15μg concentration prevent UV rays induced DNA fragmentation in submarine agarose gel electrophoresis which provides same protection when compared to standard antioxidant BHA (400μM). The cytotoxicity studies showed that, the extract and BHA provides a protection of 54% whereas, the extract of Mint leaves extract showed 72%. In conclusion, the Mint leaves extract showed a promising DNA protectant activity against UV rays induced DNA damage.
In this project, in vitro absorption enhancement activity of P-gp substrates Fexofenadine (Fx) and Ciprofloxacin (Cp) were evaluated in everted rat gut sac model and Caco-2 cell lines. Verapamil was used as P-gp inhibitor. Piper betel, Trachyspermum ammi, Plumbago zeylanica, Trikatu, Moringaoleifera, Murraya koenigii, Ferulafoitida Zingiber officinale, Cheilocostus speciosus, Capsicum frutescens Operculina turpethum Holarrhena antidysenterica Mesuaferrea, Tinospora cordifolia, and Picrorhiza kurroa, were selected and extracted with 99% alcohol and fresh juices of Citrus limon, Punica granatum seeds were also studied. In-vitro studies depicted that Fexofenadine and Ciprofloxacin absorption was increased greater than 20% in the presence of Operculinaturpethum, Capsicum frutescens, Holarrhena Antidysenterica, Tinospora cordifolia, Trikatu, Trachyspermum ammi, Plumbago zeylanica. The flux of the ciprofloxacin transport was in the range of 9-23 mcg/min and Papp 2.6 × 10-5 cm/sec to 4.1 × 10-5 cm/sec whereas Fexofenadine flux was in the range of 2-7.7 mcg/min and Papp 4.16 × 10–6 cm/sec to 1.62 × 10-5 cm/sec. In vitro antimicrobial activity of ciprofloxacin on selected microbes in presence of extracts also depicted synergistic activity. Histological studies revealed that there is no significant variation observed in the isolated sac in presence of the extracts. CaCo2 cell lines studies showed that, formulation enhanced the absorption of fexofenadine greater than 50%. Tablets were prepared and evaluated using the plant extracts which yielded >20% absorption enhancement of the substrates. In conclusion, tablet formulation containing the alcoholic extracts of Trachyspermum ammi, Plumbago zylanicum, Capsicum frutescens, Operculina turpethum, Holarrhena Antidysenterica, Tinospora cordifolia and Trikatu can act as an absorption enhancer for fexofenadine and ciprofloxacin. The mechanism of action of these herbs could be due to P-gp inhibition. Further clinical studies are needed to prove its efficacy in humans.
Nanotechnology is the branch of science and engineering accomplished by design, synthesis, characterisation, and application of materials or devices in the nanometer scale. It permits identification, development and establishes the use of material frameworks, devices and technologies withs unique properties and functions from their structures at the Subatomic, atomic, molecular as well as supermolecular levels. In recent decades, nanotechnology has attracted unparalleled attention in broad areas of research. It has been used in various fields like Engineering and Technology, Material Science, Agriculture, Medicine, Pharmacy, Biotechnology and other areas of Life science. Nanoparticles have been playing a promising role in the advancement of diagnosis and therapeutics of many health issues. They are providing the way for new approaches of drug delivery to target specific sites and achieve enhanced bioavailability. In this review, we provide an overview of the use of Metallic nanoparticles, lipid-based nanoparticles, Charge reverse nanoparticulate systems, Carbon nanotubes, Magnetic, Controlled release nanoparticles, Herbal nanoparticles etc., in Various biomedical fields like Drug delivery, Gene therapy, Tissue regeneration, Implants, Diagnosis, Imaging and treatment of various diseases. Even though numerous nanoparticulate systems were developed, only a few of them are transformed into practical use in clinical environments. Hence, focus on the development and commercialisation of nanoparticulate system is entailed.
Microorganisms are raising resistance against available antibiotics due to usage of antibiotics in a wrong way. There is lot of demand for finding /searching new natural antimicrobial agents as they are inexpensive, easily available, most of them are edible and mainly non-toxic even at the rate of milligram quantity. The aim was to study the efficiency of the anti-bacterial effect of Polyphenols enriched extract of Drum stick plant leaves (Moringa oleifera) against staphylococcus aureus. The materials involved in this study include Drum stick plant leaves, micro-organism staphylococcus aureus in the bacterial type culture collection, agar, and blood-agar plates. At 10% concentration, of Polyphenols enriched extract of Drum stick leaves had zero anti-bacterial activity, while between 20 to 25% concentrations revealed high activity against the bacteria. Thus, increased in the anti-bacterial activity was promising as the concentration augmented from 20 to 25%. The results acquired from this study points that polyphenol enriched extract of Drum stick leaves (Moringa oleifera) had antibacterial property against Staphylococcus aureus when obtained to a necessary concentration.
The global prevalence of diabetes mellitus is increasing day by day. Despite using synthetic anti-diabetic agents, diabetic patients must modify their lifestyle, including routine diet. Vegetables are the adequate source of vitamins, dietary fibres, minerals and Phytoconstituents. Use of vegetables is growing among the people as a part of the diet. They, with their antioxidant properties, can maintain good health and reduce the risk of chronic diseases. Besides, they contain many dietary fibres that are anti-diabetic. The constituents present in these vegetables help to reduce blood glucose level through several mechanisms such as alpha-amylase and alpha-glucosidase enzyme inhibition, Dipeptidyl peptidase IV (DPP IV) inhibition, enhancing the expression of peroxisome proliferator activator receptor gamma (PPAR) γ and glucose transporter 4 (GLUT4). Therefore the people must consume such vegetables with the proper knowledge to control diabetes mellitus and its complications. Hence the present review focuses on summarizing in vitro and in vivo anti-diabetic activity of most common dietary vegetables.
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