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In this study, the fluorescein dye was dissolved in the solvent Dimethyl Form amide (DMF). Weighed equivalent to 0.0664, 0.0996 and 0.132 gm from the material. Using 5mM of solvent, each weight of the substance was dissolved separately to obtain the concentration 60 and 80 mM respectively. The effects of both concentration and gamma radiation on optical spectrum of all samples were investigated at room temperature. After irradiation and within the different concentration percentage at fix dose (4.7 KGy), where the results showed increase absorbance with increase concentration (40, 60 and 80 mM). Also, it is found that the decrease absorbance spectrums at wavelength 460 and 487 nm with increase dose and the good stability absorbance spectrum with time. Because of this, the structures in question are regarded as an efficient material for carrying out real-time gamma radiation dosimetry at ambient temperature.

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