In Tunisia, the mosquito Culex pipiens shows various organophosphate resistance alleles at Ester and ace-1 loci. The characterization and the distribution pattern of these alleles were studied among 20 populations sampled from north to center of Tunisia. At the Ester locus, Ester4, Ester5, and Ester(B12) were present. A new esterase characterized by the same electrophoretic migration as esterase A1 was identified: A13, encoded by Ester(A13) allele. At the ace-1 locus, the presence of the ace-1(R), ace-1(D), and F290V mutated alleles was also detected. A large heterogeneity in allelic frequencies at Ester and ace-1 loci was observed among samples, with a high significant genotypic differentiation considering both loci (F, = 0.077, P < 10(-5)), depicting variations of insecticide treatment intensity between areas. A comparison between populations collected in 1996 and 2005 showed an absence of significant resistance evolution. However, the high frequencies of resistance alleles in 2005 populations suggested that the selection pressures are still important in Tunisia. Strategies for resistance management are discussed in the context of the current knowledge of the Tunisian situation.
Objective
To examine the effects of urbanization on the resistance status of field populations of
Culex pipiens pipiens
to organophosphate insecticide.
Methods
Bioassays and biochemical assays were conducted on Tunisian field populations of
Culex pipiens pipiens
collected in four various areas differing in the degree of urbanization. Late third and early fourth larvae were used for bioassays with chlorpyrifos and adults mosquitoes for biochemical assays including esterase and acetyl cholinesterase (AChE) activities.
Results
The distribution of resistance ratios in this study appears to be influenced by the degree of urbanization. The highest resistance was recorded in the population from most urbanized areas in Tunisia whereas the lowest resistance was found in relatively natural areas. Both metabolic and target site mechanisms were involved in the recorded resistance.
Conclusion
This is the first study in Tunisia showing evidence of the impact of urbanization on the resistance level in
Culex pipiens pipiens
. Proper management of the polluted breeding sites in the country and effective regulation of water bodies from commercial and domestic activities appear to be critical for managing insecticide resistance.
Background
Mosquitoes are considered as the main groups of arthropods that cause nuisance and public health problems.
Objectives
Evaluation of resistance to temephos insecticide in
Culex pipiens pipiens
larvae collected from three districts of Tunisia.
Methods
Late third and early fourth instars larvae of
Culex pipiens pipiens
were collected in three localities of Northern and Southern Tunisia. Field collected populations were tested against temephos insecticide and compared to bioassays of a susceptible reference strain. The cross-resistance between temephos and propoxur, and the polymorphism of over-produced esterases and AChE 1 were investigated.
Results
Studied populations exhibited tolerance to temephos with low and high levels of resistance. The resistance ratio (RR
50
) values of temephos ranged from 1.34 to 114. Synergists and starch electrophoresis showed that the metabolic resistances were involved in the recorded resistance. Likewise, the resistant target site (acetyl cholinesterase: AChE 1) was responsible for the recorded resistance to temephos compound in
Culex pipiens pipiens
.
Conclusion
The low and high resistance recorded to temephos insecticides is particularly interesting, because it leaves a range of tools useable by vector control services. However, further studies are needed to determine its spread and anticipate vector control failure where these insecticides are used.
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