ABSTRACT. Endophytic bacteria isolated from Eichhornia crassipes (Mart) Solms., collected in oil contaminated wastewater of effluent generated by Petrobras refinery in Manaus were investigated to determine their potential for producing biosurfactants. Assay with 2.6-dichlorophenol indophenol (DCPIP) indicator to verify hydrocarbon biodegradation activity; oil emulsification test; drop-collapse method; surface tension and growth curve of biosurfactant production. The M87 Microbacterium sp. strain chosen for this work was identified by the sequencing of the rDNA region and the . The different techniques of chemical analysis used for the identification of the biosurfactant isolate indicated that this is probably a long -chain fatty acid lipid type, which may be used in the future as both biosurfactant in decontamination processes of hydrocarbon-polluted areas or as bioemulsifier in countless processes, since it exhibited no toxicity as determined by Alamar Blue assay.Keywords: bioremediation, Eichhornia, diesel oil.Biossurfactantes produzidos por Microbacterium sp., isolada de macrófita aquática em área impactada por hidrocarbonetos no rio Negro, Manaus, Amazonas RESUMO. Foram investigadas bactérias endofíticas isoladas de Eichhornia crassipes (Mart.) Solms., coletadas em águas contaminadas com resíduos de petróleo em um afluente da refinaria da Petrobrás/Manaus, para avaliação da produção de biossurfactantes. Para selecionar o micro-organismos à produção e caracterização de biossurfactantes, foram realizados os seguintes testes: a descoloração do indicador 2,6 indofenol (DCPIP), emulsificação do diesel, colapso da gota, tensão superficial e curva de produção. A caracterização química foi realizada por meio das técnicas de FT-IR, UFLC/MS e RMN1H. A bactéria M87 Microbacterium neste estudo, foi identificada pelo sequenciamento da região rDNA e produziu 3g L -1 de biossurfactantes utilizando o diesel como fonte de carbono, mostrando-se eficiente na ação biodegradadora do petróleo, por meio do teste de Indofenol (DCPIP). As frações obtidas, mostraram-se eficazes na redução da tensão superficial da água abaixo de 40 mN m -1 , com destaque para a fração 1 que reduziu a tensão superficial para 34,17 mN m -1 . Pelas análises química utilizadas, pode-se inferir que, provavelmente, se trata de um ácido graxo de cadeia longa, que pode ser utilizado futuramente tanto como biossurfactante em processos de descontaminação de ambientes impactados por hidrocarbonetos, assim como bioemulsificante em inúmeros processos uma vez que não apresentou toxicidade por meio do teste realizado.Palavras-chave: biorremediação, Eichhornia, diesel.
The aim of this study was to characterize Caramuri Pouteria elegans (A.DC.) Baehni, an exotic fruit of the Amazonian biome to highlight it is a new source nutritional potential. The nutritional (vitamin A, B, C, and E) and volatile composition of the fruits was determined by physical [mass, diameter (longitudinal and transverse), yield] and chemical characterization (pH, acidity, soluble solids, proximate and mineral compositions) using headspace solid-phase microextraction (HS-SPME) by gas chromatography-mass spectrometry (GCMS). The amount of vitamin C, potassium, and magnesium detected per 100 g of sample was 143.08 mg, 97.50 mg, and 14.75 mg, respectively. Volatile profile by HS-SPME revealed 87 peaks, however, only 26 peaks were noted due to the small peak area (<1.0). Arithmetic and Kovats retention indexes were calculated for compound(s) identification. The percentage of volatiles identification was 88.46%. The majority compound was α-Pinene with 21.77%. The study reveals that Caramuri is a great source of vitamins and minerals, especially vitamin C.
PHA is a biodegradable and biocompatible natural thermoplastic produced from renewable bioresources and is hence attracting attention as a plastic material for use in the environment and medical fields. In the present study, the Lynisibacillus sp. strain UEA-20.171 was selected for production of polyhydroxyalkanoate (PHA) in bioreactor. The accumulation of polymer in the bacterium occurs when there is an imbalance in the concentration of nitrogen in relation to carbon (glucose). After the period of adaptation in mineral medium from 12 to 24 h, the cell division occurred from 12 to 24 h, and the formation of biopolymers from 36 to 48 h. It was observed that the polymer produced by the isolated UEA-20.171, was P(3HB).
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