White adipose tissue is a source of mesenchymal stromal/stem cells (MSCs) that are actively studied for their possible therapeutic use in bone tissue repair/remodeling. To better appreciate the osteogenic potential of these cells, we compared some properties of MSCs from human subcutaneous adipose tissue [subcutaneous-adipose stromal cells (S-ASCs)] and dental pulp stem cell (DPSCs) of third-impacted molars, the latter representing a well-established MSC source. Both undifferentiated cell types showed similar fibroblast-like morphology and mesenchymal marker expression. However, undifferentiated S-ASCs displayed a faster doubling time coupled to greater proliferation and colony-forming ability than DPSCs. Also, the osteogenic differentiation of S-ASCs was greater than that of DPSCs, as evaluated by the higher levels of expression of early osteogenic markers Runt-related transcription factor-2 (RUNX2) and alkaline phosphatase at days 3-14 and of extracellular matrix mineralization at days 14-21. Moreover, S-ASCs showed a better colonization of the titanium scaffold. In addition, we investigated whether S-ASC osteogenic commitment was enhanced by adenosine A1 receptor (A1R) stimulation, as previously shown for DPSCs. Although A1R expression was constant during DPSC differentiation, it increased in S-ASC at day 21 from osteogenesis induction. Accordingly, A1R stimulation by the agonist 2-chloro-N-cyclopentyl-adenosine, added to the cultures at each medium change, stimulated proliferation only in differentiating DPSC and enhanced the osteogenic differentiation earlier in DPSCs than in S-ASCs. These effects were counteracted by cell pretreatment with a selective A1R antagonist. Thus, our findings suggest that S-ASCs could be advantageously used in regenerative orthopedics/dentistry, and locally released or exogenously added purines may play a role in bone repair/remodeling, even though this aspect should be more thoroughly evaluated.
Monitoring infrastructures is becoming an important and challenging issue. In Italy, the heritage consists of more than 60,000 bridges, which need to be inspected and detected in order to guarantee their strength and durability function during nominal lifespan. In this paper, a non-destructive survey methodology for study concrete bridges surface deterioration and viaducts is presented. Terrestrial and unmanned aerial vehicle (UAV) photogrammetry has been used for visual inspection of a standard concrete overpass in L’Aquila (Italy). The obtained orthomosaic has been processed by means of Object-Based Image Analysis (OBIA) to identify and classify deteriorated areas and decay forms. The results show a satisfactory identification and survey of deteriorated areas. It has also been possible to quantify metric information, such as width and length of cracks and extension of weathered areas. This allows to perform easy and fast periodic inspections over time in order to evaluate the evolution of deterioration and plan urgency of preservation or maintenance measures.
The rapid maturation of the osteoblast-like cells on FCC titanium surface suggests that this structure could play a central role during initial phases of bone healing processes.
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