Abbreviations:ACN -acetonitrile; GH -glycoside hydrolase; LC/MS -liquid chromatography/mass spectrometry; MS -mass spectrometry; PGA -polygalacturonic acid; PL -polysaccharide lyase.

Agro-industrial wastes are potential starting materials for the production of useful value-added compounds, including prebiotic oligosaccharides. In this paper, we evaluated the potential of thermophilic bacterial pectin-and xylandegrading recombinant enzymes for the degradation of the agro-industrial wastes: apple pomace, wheat straw, wheat bran and distillers grains. For the immobilization of pectate lyase and xylanase, three different supports were used. The effect of enzyme immobilization was analyzed in terms of enhanced thermostability and activity against these wastes. For xylanase, the highest thermostability was achieved by immobilization on Sepabeads EC-EP/M. The best activity against bran and grains was obtained by immobilization on Sepabeads EC-HA/M. For pectate lyase, the highest thermostability was achieved by immobilization on Sepabeads EC-EP/M, however, activity against apple pomace pectin was slightly reduced by this immobilization. The length of oligosaccharides produced by both free and immobilized enzymes was also determined.

Geobacillus thermodenitrificans DSM 101594 was isolated as a producer of extracellular thermostable pectic polysaccharide degrading enzymes. The completely sequenced genome was 3.6 Mb in length with GC content of 48.86%. A number of genes encoding enzymatic active against the high molecular weight polysaccharides of potential biotechnological importance were identified in the genome.

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